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IP (Immunoprecipitation)
(Immunoprecipitating CD247 in jurkat whole cell lysateLane 1: Rabbit control IgG instead of AAA232758 in jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA232758 (8ug) + jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (10ug))
Rabbit anti-Human CD247 Polyclonal Antibody | anti-CD247 antibody
CD247 Antibody
Gene Names
CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
CD247, Antibody; CD247 Antibody; T-cell surface glycoprotein CD3 zeta chain (T-cell receptor T3 zeta chain) (CD antigen CD247), CD247, CD3Z T3Z TCRZ; anti-CD247 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Applicable Applications for anti-CD247 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant Human T-cell surface glycoprotein CD3 zeta chain protein (88-153AA)
Conjugate
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Species
Human
Research Area
Immunology
Target Names
CD247
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1124 / sc-20919
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating CD247 in jurkat whole cell lysateLane 1: Rabbit control IgG instead of AAA232758 in jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA232758 (8ug) + jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating CD247 in jurkat whole cell lysateLane 1: Rabbit control IgG instead of AAA232758 in jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA232758 (8ug) + jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (10ug))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232758 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA232758 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human tonsil tissue using AAA232758 at dilution of 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human tonsil tissue using AAA232758 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human lymph node tissue using AAA232758 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human lymph node tissue using AAA232758 at dilution of 1:100)
WB (Western Blot)
(Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: CD247 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 19 KDaObserved band size: 19 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Jurkat whole cell lysateAll lanes: CD247 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 19 KDaObserved band size: 19 KDa)
Related Product Information for anti-CD247 antibody
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD3 zeta chain isoform 2
NCBI Official Synonym Full Names
CD247 molecule
NCBI Official Symbol
CD247
NCBI Official Synonym Symbols
T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
NCBI Protein Information
T-cell surface glycoprotein CD3 zeta chain
UniProt Protein Name
T-cell surface glycoprotein CD3 zeta chain
UniProt Gene Name
CD247
UniProt Synonym Gene Names
CD3Z; T3Z; TCRZ
UniProt Entry Name
CD3Z_HUMAN
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Product Notes
The CD247 cd247 (Catalog #AAA232758) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD247 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD247 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CD247 cd247 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD247, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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