IHC (Immunohistochemisry)
(Anti-ALK antibody, AAA45002, IHC(P)IHC(P): Human Rectal Cancer Tissue)
Rabbit ALK Polyclonal Antibody | anti-ALK antibody
Anti-ALK antibody
Gene Names
ALK; CD246; NBLST3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
ALK, Antibody; Anti-ALK antibody; ALK tyrosine kinase receptor; anaplastic lymphoma receptor tyrosine kinase; Alk antibody; ALK tyrosine kinase receptor antibody; ALK tyrosine kinase receptor precursor antibody; ALK/EML4 fusion gene, included antibody; ALK/NPM1 fusion gene, included antibody; ALK_HUMAN antibody; anaplastic lymphoma kinase(Ki-1) antibody; Anaplastic lymphoma kinase antibody; Anaplastic lymphoma kinase Ki 1 antibody; Anaplastic lymphoma kinase Ki1 antibody; Anaplastic lymphoma kinase p80 antibody; anaplastic lymphoma receptor tyrosine kinase antibody; CD 246 antibody; CD246 antibody; CD246 antigen antibody; EC 2.7.10.1 antibody; Ki 1 antibody; Ki1 antibody; mutant anaplastic lymphoma kinase antibody; NBLST 3 antibody; NBLST3 antibody; Tcrz antibody; TFG/ALK antibody; anti-ALK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
1620
Applicable Applications for anti-ALK antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ALK (1571-1589aa LFRLRHFPCGNVNYGYQQQ), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti-ALK antibody, AAA45002, IHC(P)IHC(P): Human Rectal Cancer Tissue)
IHC (Immunohistochemisry)
(Anti-ALK antibody, AAA45002, IHC(P)IHC(P): Human Rectal Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-ALK antibody, AAA45002, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti-ALK antibody, AAA45002, IHC(P)IHC(P): Rat Intestine Tissue)
WB (Western Blot)
(Anti-ALK antibody, AAA45002, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: U87 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: JURKAT Cell Lysate)
WB (Western Blot)
(Anti-ALK antibody, AAA45002, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: U87 Cell LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: JURKAT Cell Lysate)
Related Product Information for anti-ALK antibody
Description: Rabbit IgG polyclonal antibody for ALK tyrosine kinase receptor(ALK) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: ALK(Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246(cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.
Background: ALK(Anaplastic lymphoma kinase) also known as ALK tyrosine kinase receptor or CD246(cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. The ALK gene is mapped on 2p23.2-p23.1. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of ALK expression in ALK-mutated cells, but also in cell lines overexpressing a wildtype ALK, led to a marked decrease of cell proliferation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
176,442 Da
NCBI Official Full Name
ALK tyrosine kinase receptor
NCBI Official Synonym Full Names
anaplastic lymphoma receptor tyrosine kinase
NCBI Official Symbol
ALK
NCBI Official Synonym Symbols
CD246; NBLST3
NCBI Protein Information
ALK tyrosine kinase receptor; CD246 antigen; mutant anaplastic lymphoma kinase
UniProt Protein Name
ALK tyrosine kinase receptor
UniProt Gene Name
ALK
UniProt Entry Name
ALK_HUMAN
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Product Notes
The ALK alk (Catalog #AAA45002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALK antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ALK can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ALK alk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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