IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded Skeletal Muscle using alpha Actin antibody)
Rabbit alpha Actin Polyclonal Antibody | anti-ACTA1 antibody
alpha Actin antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
Applications
Western Blot, Immunohistochemistry
Purity
alpha Actin antibody was purified by antigen-affinity chromatography
Synonyms
alpha Actin, Antibody; alpha Actin antibody; Polyclonal alpha Actin; Anti-alpha Actin; actin alpha 1 skeletal muscle; ACTA; ASMA; NEM2; ACTA1; actin alpha skeletal muscle; CFTD; NEM1; alpha skeletal muscle actin; CFTD1; NEM3; MPFD; alpha-actin-1; CFTDM; anti-ACTA1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
alpha Actin antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.81 mg/ml (varies by lot)
Sequence Length
377
Applicable Applications for anti-ACTA1 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Biological Significance
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Cross-Reactivity
Human, Mouse
Immunogen
alpha Actin antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 377 of alpha Actin
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded Skeletal Muscle using alpha Actin antibody)
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded Skeletal Muscle using alpha Actin antibody)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:Raji) using a 10 % SDS PAGE gel and alpha Actin antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:Raji) using a 10 % SDS PAGE gel and alpha Actin antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1; D: C2C12) using a 10 % SDS PAGE gel and alpha Actin antibody at a dilution of 1:5000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC; C: BCL-1; D: C2C12) using a 10 % SDS PAGE gel and alpha Actin antibody at a dilution of 1:5000)
Related Product Information for anti-ACTA1 antibody
Affinity purified Rabbit polyclonal alpha Actin antibody
Product Categories/Family for anti-ACTA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42 kDa
NCBI Official Full Name
alpha-actin
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
NCBI Protein Information
actin, alpha skeletal muscle
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
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Product Notes
The ACTA1 acta1 (Catalog #AAA107830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's alpha Actin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ACTA1 acta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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