WB (Western Blot)
(Detection of human and mouse alpha Actinin 4/ACTN4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-alpha Actinin 4/ACTN4 antibody AAA212923 (lot AAA212923-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse alpha Actinin 4/ACTN4 Polyclonal Antibody | anti-ACTN4 antibody
Rabbit anti-alpha Actinin 4/ACTN4 Antibody, Affinity Purified
Gene Names
ACTN4; FSGS; FSGS1; ACTININ-4
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
alpha Actinin 4/ACTN4, Antibody; Rabbit anti-alpha Actinin 4/ACTN4 Antibody, Affinity Purified; focal segmental glomerulosclerosis 1; non-muscle alpha-actinin 4; ACTININ-4; FSGS; alpha-actinin-4; FSGS1; Alpha-actinin-4; actinin alpha 4; anti-ACTN4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ACTN4 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse alpha Actinin 4/ACTN4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-alpha Actinin 4/ACTN4 antibody AAA212923 (lot AAA212923-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse alpha Actinin 4/ACTN4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-alpha Actinin 4/ACTN4 antibody AAA212923 (lot AAA212923-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human alpha Actinin 4/ACTN4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-alpha Actinin 4/ACTN4 antibody AAA212923 (lot AAA212923-1) used for IP at 6 ug per reaction. For blotting immunoprecipitated alpha Actinin 4/ACTN4, AAA212923 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human alpha Actinin 4/ACTN4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-alpha Actinin 4/ACTN4 antibody AAA212923 (lot AAA212923-1) used for IP at 6 ug per reaction. For blotting immunoprecipitated alpha Actinin 4/ACTN4, AAA212923 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-ACTN4 antibody
Alpha Actinin 4 /ACTN4 is an F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). It is probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). It is involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. It links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). alpha Actinin 4 /ACTN4 may also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43707].
Product Categories/Family for anti-ACTN4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105kDa
NCBI Official Full Name
alpha-actinin-4
NCBI Official Synonym Full Names
actinin, alpha 4
NCBI Official Symbol
ACTN4
NCBI Official Synonym Symbols
FSGS; FSGS1; ACTININ-4
NCBI Protein Information
alpha-actinin-4; actinin alpha4 isoform; non-muscle alpha-actinin 4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1
UniProt Protein Name
Alpha-actinin-4
UniProt Gene Name
ACTN4
UniProt Entry Name
ACTN4_HUMAN
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Product Notes
The ACTN4 actn4 (Catalog #AAA212923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-alpha Actinin 4/ACTN4 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Actinin 4/ACTN4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ACTN4 actn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Actinin 4/ACTN4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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