WB (Western Blot)
(Western blot analysis of alpha-Actinin in lysates of C6, using alpha-Actinin Antibody(AF4629).)
Rabbit alpha-Actinin Polyclonal Antibody | anti-ACTN1 antibody
alpha-Actinin Antibody
Gene Names
ACTN1; BDPLT15
Reactivity
Human, Mouse, RatPredicted Reactivity: Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
alpha-Actinin, Antibody; alpha-Actinin Antibody; actinin 1 smooth muscle; Actinin alpha 1; actinin, alpha 1; ACTN 1; Actn1; ACTN1_HUMAN; Alpha Actinin 1; Alpha actinin cytoskeletal isoform; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; BDPLT15; F actin cross linking protein; F-actin cross-linking protein; FLJ40884; FLJ54432; Non muscle alpha actinin 1; Non-muscle alpha-actinin-1; anti-ACTN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
alpha-Actinin Antibody detects endogenous levels of total alpha-Actinin.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ACTN1 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human alpha-Actinin, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Homodimer; antiparallel. Interacts with MYOZ2, TTID and LPP. Interacts with DDN. Interacts with PSD. Interacts with MICALL2 (By similarity). Interacts with DNM2 and CTTN. Interacts with PDLIM1. Interacts with PDLIM2. Interacts with PDLIM4 (via PDZ domain) (By similarity).
Similarity
Belongs to the alpha-actinin family.
Subcellular Location
Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril>Sarcomere>Z line. Cell membrane. Cell junction. Cell projection>Ruffle.
Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures.
Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of alpha-Actinin in lysates of C6, using alpha-Actinin Antibody(AF4629).)
WB (Western Blot)
(Western blot analysis of alpha-Actinin in lysates of C6, using alpha-Actinin Antibody(AF4629).)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using alpha-Actinin Antibody.Lane 1: NIH-3T3 cells, blocked with antigen-specific peptides,Lane 2: NIH-3T3 cells,Lane 3: MDA-MB-231 cells,Lane 4: EC304 cells.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using alpha-Actinin Antibody.Lane 1: NIH-3T3 cells, blocked with antigen-specific peptides,Lane 2: NIH-3T3 cells,Lane 3: MDA-MB-231 cells,Lane 4: EC304 cells.)
Related Product Information for anti-ACTN1 antibody
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,058 Da
NCBI Official Full Name
alpha-actinin-1 isoform b
NCBI Official Synonym Full Names
actinin, alpha 1
NCBI Official Symbol
ACTN1
NCBI Official Synonym Symbols
BDPLT15
NCBI Protein Information
alpha-actinin-1; actinin 1 smooth muscle; non-muscle alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform
UniProt Protein Name
Alpha-actinin-1
UniProt Gene Name
ACTN1
UniProt Entry Name
ACTN1_HUMAN
Similar Products
Product Notes
The ACTN1 actn1 (Catalog #AAA329509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha-Actinin Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-Actinin can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the ACTN1 actn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-Actinin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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