WB (Western Blot)
(Western blot analysis of different tissue lysates using rabbit pAb to alphaII-spectrin, AAA76732, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact alphaII-spectrin.)
Rabbit alpha-II-Spectrin/alpha-Fodrin Polyclonal Antibody | anti-SPTAN1 antibody
alpha-II-Spectrin/alpha-Fodrin
Gene Names
SPTAN1; NEAS; EIEE5; SPTA2
Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Synonyms
alpha-II-Spectrin/alpha-Fodrin, Antibody; alpha-II-Spectrin/alpha-Fodrin; anti-SPTAN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Liquid. Supplied as an aliquot of serum plus 5mM NaN3
Applicable Applications for anti-SPTAN1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Recombinant constructs spanning most of human alpha-II spectrin expressed in and purified from E coli
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of different tissue lysates using rabbit pAb to alphaII-spectrin, AAA76732, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact alphaII-spectrin.)
WB (Western Blot)
(Western blot analysis of different tissue lysates using rabbit pAb to alphaII-spectrin, AAA76732, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact alphaII-spectrin.)
IF (Immunofluorescence)
(Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with rabbit pAb to alphaII-spectrin, AAA76732, dilution 1:500 in green, and costained with mouse mAb to Ankyrin3 in red. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains submembranous cytoskeleton of neuronal cells. The Ankyrin3 antibody labels axonal initial segments of neurons.)
IF (Immunofluorescence)
(Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with rabbit pAb to alphaII-spectrin, AAA76732, dilution 1:500 in green, and costained with mouse mAb to Ankyrin3 in red. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains submembranous cytoskeleton of neuronal cells. The Ankyrin3 antibody labels axonal initial segments of neurons.)
Related Product Information for anti-SPTAN1 antibody
Spectrin family molecules are important high molecular weight components of the submembranous cytoskeleton of eukaryotic cells. These proteins were isolated originally from lysed red blood cell membrane preparations which were named "ghosts", which gave rise to the name spectrin. They function as major components of the membraneous cytoskeleton, mediating interactions between integral membrane proteins, actin and many other cellular components. This antibody binds specifically to alphaII-spectrin, also known as non-erythroid spectrin or fodrin. In the CNS this protein is expressed only in neurons and so the antibody can be used to reveal the submembranous neuronal cytoskeleton in IF, ICC and IHC.
It was made against the recombinant human protein construct derived from the C-terminus of alphaII-spectrin comprising the C-terminal 14 spectrin repeats, specifically amino acids 676-2447. This antibody can be used to study alphaII-spectrin on western blots and to visualize the neuronal plasma membrane cytoskeleton in cells in culture and sectioned material.
It was made against the recombinant human protein construct derived from the C-terminus of alphaII-spectrin comprising the C-terminal 14 spectrin repeats, specifically amino acids 676-2447. This antibody can be used to study alphaII-spectrin on western blots and to visualize the neuronal plasma membrane cytoskeleton in cells in culture and sectioned material.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
284,539 Da
NCBI Official Full Name
spectrin alpha chain, non-erythrocytic 1 isoform 1
NCBI Official Synonym Full Names
spectrin, alpha, non-erythrocytic 1
NCBI Official Symbol
SPTAN1
NCBI Official Synonym Symbols
NEAS; EIEE5; SPTA2
NCBI Protein Information
spectrin alpha chain, non-erythrocytic 1; alpha-fodrin; alpha-II spectrin; fodrin alpha chain; spectrin, non-erythroid alpha chain; spectrin, non-erythroid alpha subunit
UniProt Protein Name
Spectrin alpha chain, non-erythrocytic 1
UniProt Gene Name
SPTAN1
UniProt Synonym Gene Names
NEAS; SPTA2
UniProt Entry Name
SPTN1_HUMAN
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Product Notes
The SPTAN1 sptan1 (Catalog #AAA76732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha-II-Spectrilpha-Fodrin reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-II-Spectrilpha-Fodrin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the SPTAN1 sptan1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-II-Spectrin/alpha-Fodrin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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