Rabbit Alpha-Tubulin Polyclonal Antibody

Alpha-Tubulin Antibody, Rabbit PAb, Antigen Affinity Purified

Cat. Num. AAA258757

• Reactivity: Human, Mouse, Rat, Rabbit, Rhesus, Pig, Chinese hamster, Bovine, Dog; (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Alpha-Tubulin, Antibody; Alpha-Tubulin Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-B-ALPHA-1 Antibody; Anti-LIS3 Antibody; Anti-TUBA3 Antibody; anti-Alpha-Tubulin antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Rabbit, Rhesus, Pig, Chinese hamster, Bovine, Dog; (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Alpha-Tubulin antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Alpha-Tubulin
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Alpha-Tubulin, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-Alpha-Tubulin rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysate Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:50 kDaObserved band size:50 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of KLH-SMCCA1235 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA1235 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

IP (Immunoprecipitation)

(Alpha-Tubulin was immunoprecipitated using:Lane A:0.5 mg NIH 3T3 Whole Cell LysateLane B:0.5 mg Mouse brain Whole Cell Lysate4 uL anti-Alpha-Tubulin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-Alpha-Tubulin rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 50 kDaObserved band size :50 kDa)

Related Product Information for anti-Alpha-Tubulin antibody

TUBA1A is a highly-conserved gene with few changes among eukaryotes and few polymorphic variants in human populations. TUBA1A encodes the tubulin alpha-1A chain, a protein that is highly expressed in the cerebral cortex, hippocampus, cerebellum, and brainstem of the developing fetal brain, with a decrease in postnatal and adult stages. Alpha- and beta-tubulin subunits form dimers, which then co-assemble into microtubule polymers: dynamic, scaffold-like structures that perform key functions during neurogenesis, neuronal migration, and cortical organization. Microtubules are dynamic polymers that perform a range of mechanical tasks within the cell. Mutations in TUBA1A have been reported to cause a range of brain malformations.

References

Gardner JF, et al. (2018) Clinical and functional characterization of the recurrent tuba1a p.(arg2his) mutation. Brain Sci 8 (8): 145.