IHC (Immunohiostchemistry)
(ALPP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human ALPP Polyclonal Antibody | anti-ALPP antibody
ALPP Antibody (Center)
Gene Names
ALPP; ALP; PALP; PLAP; PLAP-1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ALPP, Antibody; ALPP Antibody (Center); Alkaline phosphatase; placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; ALPP; PLAP; anti-ALPP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ALPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-309 amino acids from the Central region of human ALPP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.29 (varies by lot)
Sequence Positions
282-309
Sequence Length
535
Applicable Applications for anti-ALPP antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(ALPP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ALPP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(ALPP Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).)
WB (Western Blot)
(ALPP Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow).)
Related Product Information for anti-ALPP antibody
There are at least four distinct but related alkaline
phosphatases: intestinal, placental, placental-like, and
liver/bone/kidney (tissue non-specific). The first three are
located together on chromosome 2 while the tissue non-specific form
is located on chromosome 1. The product of this gene is a membrane
bound glycosylated enzyme, also referred to as the heat stable
form, that is expressed primarily in the placenta although it is
closely related to the intestinal form of the enzyme as well as to
the placental-like form. The coding sequence for this form of
alkaline phosphatase is unique in that the 3' untranslated region
contains multiple copies of an Alu family repeat. In addition, this
gene is polymorphic and three common alleles (type 1, type 2 and
type 3) for this form of alkaline phosphatase have been well
characterized.
phosphatases: intestinal, placental, placental-like, and
liver/bone/kidney (tissue non-specific). The first three are
located together on chromosome 2 while the tissue non-specific form
is located on chromosome 1. The product of this gene is a membrane
bound glycosylated enzyme, also referred to as the heat stable
form, that is expressed primarily in the placenta although it is
closely related to the intestinal form of the enzyme as well as to
the placental-like form. The coding sequence for this form of
alkaline phosphatase is unique in that the 3' untranslated region
contains multiple copies of an Alu family repeat. In addition, this
gene is polymorphic and three common alleles (type 1, type 2 and
type 3) for this form of alkaline phosphatase have been well
characterized.
Product Categories/Family for anti-ALPP antibody
References
Stec, B., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 8), 866-870 (2010) :
Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009)
Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)
Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009)
Roberson, J.R., et al. Pediatr Blood Cancer 51(6):840-842(2008)
Wang, F., et al. Am. J. Surg. Pathol. 33(10):1529-1539(2009)
Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)
Zhu, J.F., et al. Zhonghua Wai Ke Za Zhi 47(5):381-384(2009)
Roberson, J.R., et al. Pediatr Blood Cancer 51(6):840-842(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57954
NCBI Official Full Name
alkaline phosphatase, placental type preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental
NCBI Official Symbol
ALPP
NCBI Official Synonym Symbols
ALP; PALP; PLAP; PLAP-1
NCBI Protein Information
alkaline phosphatase, placental type
UniProt Protein Name
Alkaline phosphatase, placental type
UniProt Gene Name
ALPP
UniProt Synonym Gene Names
PLAP; PLAP-1
UniProt Entry Name
PPB1_HUMAN
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Product Notes
The ALPP alpp (Catalog #AAA287729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 282-309. The ALPP Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALPP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ALPP alpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALPP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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