WB (Western Blot)
(Detection of human Aly by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Aly antibody AAA212062 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Aly was also immunoprecipitated by rabbit anti-Aly antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human Aly Polyclonal Antibody | anti-Aly antibody
Rabbit anti-Aly Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Aly, Antibody; Rabbit anti-Aly Antibody, Affinity Purified; BEF; REF; transcriptional coactivator Aly/REF; THOC4; tho4; THO complex subunit 4; THO complex 4; bZIP enhancing factor; ally of AML-1 and LEF-1; Aly/REF export factor; ALY/REF; ALY; bZIP-enhancing factor BEF; anti-Aly antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Aly antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Aly by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Aly antibody AAA212062 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Aly was also immunoprecipitated by rabbit anti-Aly antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human Aly by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Aly antibody AAA212062 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Aly was also immunoprecipitated by rabbit anti-Aly antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human Aly by immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-Aly (Cat. No. AAA212062 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Aly by immunohistochemistry. Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-Aly (Cat. No. AAA212062 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Aly antibody
Aly (ally of AML-1 and LEF-1) is a component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. Aly also functions as a chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. Additionally, Aly plays a role in mRNA processing and export and may function as scaffold that mediates interactions between proteins and/or RNA. Aly is also part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Aly directs mRNA derived from Herpes simplex virus intron-less genes to the NXF1-mediated export pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q886V81].
Product Categories/Family for anti-Aly antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Aly alyref (Catalog #AAA212062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Aly Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aly can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Aly alyref for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aly, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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