WB (Western Blot)
(Detection of human and mouse ANAPC10 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ANAPC10 antibody AAA213038 (lot AAA213038-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse ANAPC10/APC10 Polyclonal Antibody | anti-ANAPC10/APC10 antibody
Rabbit anti-ANAPC10/APC10 Antibody, Affinity Purified
Gene Names
ANAPC10; DOC1; APC10
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ANAPC10/APC10, Antibody; Rabbit anti-ANAPC10/APC10 Antibody, Affinity Purified; DOC1; cyclosome subunit 10; anaphase-promoting complex subunit 10; APC10; Anaphase-promoting complex subunit 10; anaphase promoting complex subunit 10; anti-ANAPC10/APC10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ANAPC10/APC10 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse ANAPC10 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ANAPC10 antibody AAA213038 (lot AAA213038-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse ANAPC10 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ANAPC10 antibody AAA213038 (lot AAA213038-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ANAPC10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ANAPC10 antibody AAA213038 (lot AAA213038-1) used for IP at 6 ug per reaction. ANAPC10 was also immunoprecipitated by rabbit anti-ANAPC10 antibody For blotting immunoprecipitated ANAPC10, AAA213038 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human ANAPC10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ANAPC10 antibody AAA213038 (lot AAA213038-1) used for IP at 6 ug per reaction. ANAPC10 was also immunoprecipitated by rabbit anti-ANAPC10 antibody For blotting immunoprecipitated ANAPC10, AAA213038 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-ANAPC10/APC10 antibody
ANAPC10/APC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]) [taken from NCBI Entrez Gene (Gene ID: 10393)].
Product Categories/Family for anti-ANAPC10/APC10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,252 Da
NCBI Official Full Name
anaphase-promoting complex subunit 10 isoform 1
NCBI Official Synonym Full Names
anaphase promoting complex subunit 10
NCBI Official Symbol
ANAPC10
NCBI Official Synonym Symbols
DOC1; APC10
NCBI Protein Information
anaphase-promoting complex subunit 10; cyclosome subunit 10
UniProt Protein Name
Anaphase-promoting complex subunit 10
UniProt Gene Name
ANAPC10
UniProt Synonym Gene Names
APC10; APC10
UniProt Entry Name
APC10_HUMAN
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Product Notes
The ANAPC10/APC10 anapc10 (Catalog #AAA213038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ANAPC10/APC10 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ANAPC10/APC10 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ANAPC10/APC10 anapc10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANAPC10/APC10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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