Rabbit anti-Human Androgen Receptor Polyclonal Antibody | anti-AR antibody

Androgen Receptor (Phospho-Ser650) Antibody

Cat. Num. AAA29666

• Gene Names: AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Androgen Receptor, Antibody; Androgen Receptor (Phospho-Ser650) Antibody; ANDR; DHTR; AR; anti-AR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of Androgen Receptor only when phosphorylated at serine 650.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 920

• Applicable Applications for anti-AR antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 650 (T-T-S(p)-P-T) derived from Human Androgen Receptor.
• Modification: Phospho
• Target Name: Androgen Receptor
• Preparation and Storage: Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

WB (Western Blot)

(Western blot analysis of extracts from 293 cells, treated with PMA or calf intestinal phosphatase (CIP), using Androgen Receptor (Phospho-Ser650) Antibody (AAA29666).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Androgen Receptor (Phospho-Ser650) Antibody (AAA29666) (left) or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-AR antibody

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Product Categories/Family for anti-AR antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 21322252
• NCBI GeneID: 367
• NCBI Accession #: NP_000035.2
• NCBI GenBank Nucleotide #: NM_000044.3
• UniProt Accession #: P10275; Q9UD95; A2RUN2; B1AKD7
• Molecular Weight: Predicted MW: 110kd
• NCBI Official Full Name: androgen receptor isoform 1
• NCBI Official Synonym Full Names: androgen receptor
• NCBI Official Symbol: AR
• NCBI Official Synonym Symbols: KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• NCBI Protein Information: androgen receptor
• UniProt Protein Name: Androgen receptor
• UniProt Gene Name: AR
• UniProt Synonym Gene Names: DHTR; NR3C4
• UniProt Entry Name: ANDR_HUMAN