WB (Western Blot)
(Androgen Receptor antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of LnCaP cells probed with Androgen Receptor antibody (pAb) (2 ug/ml).)
Rabbit anti-Human, Mouse Androgen Receptor Polyclonal Antibody | anti-AR antibody
Androgen Receptor antibody (pAb)
Gene Names
AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Human, Mouse
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
Androgen Receptor, Antibody; Androgen Receptor antibody (pAb); AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; anti-AR antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
PBS pH7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-AR antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This Androgen Receptor antibody was raised against a peptide in the N-terminal region of human Androgen receptor.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Androgen Receptor antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of LnCaP cells probed with Androgen Receptor antibody (pAb) (2 ug/ml).)
WB (Western Blot)
(Androgen Receptor antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of LnCaP cells probed with Androgen Receptor antibody (pAb) (2 ug/ml).)
ChIP (Chromatin Immunoprecipitation)
(Androgen Receptor antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of VCAP60 cell chromatin and 10 ug of Androgen receptor antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(Androgen Receptor antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of VCAP60 cell chromatin and 10 ug of Androgen receptor antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(Androgen Receptor antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from a human prostate cancer cell line (3 million cells). ChIP DNA was sequenced on the Illumina GAII and 22 million sequence tags were mapped to identify Androgen Receptor binding sites. The image shows hundreds of strong binding sites dispersed across the right arm of human chromosome 16.)
ChIP (Chromatin Immunoprecipitation)
(Androgen Receptor antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from a human prostate cancer cell line (3 million cells). ChIP DNA was sequenced on the Illumina GAII and 22 million sequence tags were mapped to identify Androgen Receptor binding sites. The image shows hundreds of strong binding sites dispersed across the right arm of human chromosome 16.)
Related Product Information for anti-AR antibody
Short Description: Our Androgen Receptor antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human and Mouse samples.
Background: Androgen Receptor (AR, NR3C4) is a nuclear receptor that facilitates signaling of testosterone and other androgenic hormones in the cytoplasm and translocates into the nucleus upon hormone binding. AR serves as a DNA binding activator of transcription of hormone responsive genes. There are two forms of the Androgen Receptor, the AR-A form, lacking the N-terminal 187 amino acids and the full length AR-B form. Androgen Receptor is involved in the development of both primary and secondary male sexual characteristics and abnormal expression of AR is linked to prostate cancer.
Background: Androgen Receptor (AR, NR3C4) is a nuclear receptor that facilitates signaling of testosterone and other androgenic hormones in the cytoplasm and translocates into the nucleus upon hormone binding. AR serves as a DNA binding activator of transcription of hormone responsive genes. There are two forms of the Androgen Receptor, the AR-A form, lacking the N-terminal 187 amino acids and the full length AR-B form. Androgen Receptor is involved in the development of both primary and secondary male sexual characteristics and abnormal expression of AR is linked to prostate cancer.
Product Categories/Family for anti-AR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,643 Da
NCBI Official Full Name
androgen receptor isoform 1
NCBI Official Synonym Full Names
androgen receptor
NCBI Official Symbol
AR
NCBI Official Synonym Symbols
KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
NCBI Protein Information
androgen receptor; androgen nuclear receptor variant 2; dihydrotestosterone receptor; nuclear receptor subfamily 3 group C member 4
UniProt Protein Name
Androgen receptor
UniProt Gene Name
AR
UniProt Synonym Gene Names
DHTR; NR3C4
UniProt Entry Name
ANDR_HUMAN
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Product Notes
The AR ar (Catalog #AAA59819) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Androgen Receptor antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Androgen Receptor can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the AR ar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Androgen Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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