IHC (Immunohiostchemistry)
(Staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Rabbit ANKRD28 Polyclonal Antibody | anti-ANKRD28 antibody
Phospho-ANKRD28 (Ser1007) Antibody
Gene Names
ANKRD28; PITK; PPP1R65
Reactivity
Human, MousePredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
ANKRD28, Antibody; Phospho-ANKRD28 (Ser1007) Antibody; AI465466; AI604979; ANKRD 28; Ankrd28; Ankyrin repeat domain 28; E430019N21Rik; KIAA0379; mKIAA0379; Phosphatase interactor targeting K protein; Phosphatase interactor targeting protein hnRNP K; PITK; PPP1R65; RGD1559931; anti-ANKRD28 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-ANKRD28 (Ser1007) Antibody detects endogenous levels of ANKRD28 only when phosphorylated at Ser1007.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ANKRD28 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ANKRD28 around the phosphorylation site of Ser1007.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Protein phosphatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed by the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). Interacts with PPP1C and HNRPK. Interacts with PPP6C, PPP6R1 and PPP6R3.
Subcellular Location
Nucleus>Nucleoplasm.
Note: Seems to be excluded from nucleoli.
Note: Seems to be excluded from nucleoli.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(Staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Human ganstric cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of ANKRD28 (Phospho-Ser1007) using Mouse heart tissue lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of ANKRD28 (Phospho-Ser1007) using Mouse heart tissue lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-ANKRD28 antibody
Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Selectively inhibits the phosphatase activity of PPP1C. Targets PPP1C to modulate HNRPK phosphorylation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,966 Da
NCBI Official Full Name
serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A isoform b
NCBI Official Synonym Full Names
ankyrin repeat domain 28
NCBI Official Symbol
ANKRD28
NCBI Official Synonym Symbols
PITK; PPP1R65
NCBI Protein Information
serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; PP6-ARS-A; phosphatase interactor targeting K protein; ankyrin repeat domain-containing protein 28; protein phosphatase 1, regulatory subunit 65; protein phosphatase 6 ankyrin rep
UniProt Protein Name
Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A
UniProt Gene Name
ANKRD28
UniProt Synonym Gene Names
KIAA0379; PP6-ARS-A; Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A; PITK
UniProt Entry Name
ANR28_HUMAN
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Product Notes
The ANKRD28 ankrd28 (Catalog #AAA329784) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ANKRD28 (Ser1007) Antibody reacts with Human, Mouse Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%) and may cross-react with other species as described in the data sheet. AAA Biotech's ANKRD28 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ANKRD28 ankrd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANKRD28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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