WB (Western Blot)
(Detection of human ANKRD28/PITK by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa cells (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ANKRD28/PITK antibody AAA211254 used for WB at 0.02 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody BL4320, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human ANKRD28/PITK Polyclonal Antibody | anti-ANKRD28/PITK antibody
Rabbit anti-ANKRD28/PITK Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ANKRD28/PITK, Antibody; Rabbit anti-ANKRD28/PITK Antibody, Affinity Purified; PP6-ARS-A; serine/threonine-protein phosphatase 6 regulatory subunit ARS-A; serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; protein phosphatase 6 ankyrin repeat subunit A; PPP1R65; PITK; phosphatase interactor targeting protein hnRNP K; phosphatase interactor targeting K protein; ankyrin repeat domain-containing protein 28; protein phosphatase 1, regulatory subunit 65; Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; ankyrin repeat domain 28; anti-ANKRD28/PITK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ANKRD28/PITK antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1003 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ANKRD28/PITK by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa cells (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ANKRD28/PITK antibody AAA211254 used for WB at 0.02 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody BL4320, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human ANKRD28/PITK by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa cells (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ANKRD28/PITK antibody AAA211254 used for WB at 0.02 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). ANKRD28/PITK was also immunoprecipitated by rabbit anti-ANKRD28/PITK antibody BL4320, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human ANKRD28/PITK by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ANKRD28/PITK (Cat. No. AAA211254 Lot1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ANKRD28/PITK by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ANKRD28/PITK (Cat. No. AAA211254 Lot1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-ANKRD28/PITK antibody
ANKRD28/PITK is a regulatory subunit of protein phosphatase-1 (PP1) that targets the PP1 catalytic subunit to nuclear foci to dephosphorylate and modulate the transcriptional activity of heterogeneous nuclear ribonucleoprotein K (hnRNPK). ANKRD28/PITK has been shown to be phosphorylated and regulated by calcium/calmodulin-dependent kinase II-delta (CaMKIIdelta) and glycogen synthase kinase-3 (GSK3).
Product Categories/Family for anti-ANKRD28/PITK antibody
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
UniProt Protein Name
Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A
UniProt Gene Name
ANKRD28
UniProt Synonym Gene Names
KIAA0379; PP6-ARS-A; Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A; PITK
UniProt Entry Name
ANR28_HUMAN
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Product Notes
The ANKRD28/PITK ankrd28 (Catalog #AAA211254) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ANKRD28/PITK Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANKRD28/PITK can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ANKRD28/PITK ankrd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANKRD28/PITK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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