Rabbit Annexin A1 Polyclonal Antibody | anti-ANXA1 antibody

Phospho-Annexin A1 (Tyr21) Antibody

Cat. Num. AAA329785

• Gene Names: ANXA1; ANX1; LPC1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: Annexin A1, Antibody; Phospho-Annexin A1 (Tyr21) Antibody; Annexin 1; Annexin A1; Annexin I (lipocortin I); Annexin I; Annexin-1; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANX1; ANXA 1; ANXA1; ANXA1 protein; ANXA1_HUMAN; Calpactin 2; Calpactin II; Calpactin-2; CalpactinII; Chromobindin 9; Chromobindin-9; Chromobindin9; HGNC:533; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI; LPC 1; LPC1; p35; Phospholipase A2 inhibitory protein; anti-ANXA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-Annexin A1 (Tyr21) Antibody detects endogenous levels of Annexin A1 only when phosphorylated at Tyr21.; Tissue Specificity: Detected in resting neutrophils. Detected in peripheral blood T-cells. Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors. Detected in placenta (at protein level). Detected in liver.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ANXA1 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human Annexin A1 around the phosphorylation site of Tyr21.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment. Sumoylated.
• Subunit Structure: Homodimer; non-covalently linked (By similarity). Homodimer; linked by transglutamylation. Homodimers linked by transglutamylation are observed in placenta, but not in other tissues. Interacts with S100A11. Heterotetramer, formed by two molecules each of S100A11 and ANXA1. Interacts with DYSF (By similarity). Interacts with EGFR (By similarity).
• Similarity: The full-length protein can bind eight Ca (2+) ions via the annexin repeats. Calcium binding causes a major conformation change that modifies dimer contacts and leads to surface exposure of the N-terminal phosphorylation sites; in the absence of Ca (2+), these sites are buried in the interior of the protein core. The N-terminal region becomes disordered in response to calcium-binding.The N-terminal 26 amino acids are sufficient for its extracellular functions in the regulation of inflammation and wound healing (PubMed:25664854). Acylated peptides that contain the first 26 amino acids of the mature protein can activate signaling via the formyl peptide receptors (PubMed:15187149, PubMed:25664854).Belongs to the annexin family.
• Subcellular Location: Nucleus. Cytoplasm. Cell projection>Cilium. Cell membrane. Membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein. Basolateral cell membrane. Apical cell membrane. Lateral cell membrane. Secreted. Secreted>Extracellular space. Cell membrane>Peripheral membrane protein>Extracellular side. Secreted>Extracellular exosome. Cytoplasmic vesicle>Secretory vesicle lumen. Cell projection>Phagocytic cup. Early endosome. Cytoplasmic vesicle membrane>Peripheral membrane protein.; Note: Secreted, at least in part via exosomes and other secretory vesicles. Detected in exosomes and other extracellular vesicles (PubMed:25664854). Detected in gelatinase granules in resting neutrophils (PubMed:10772777). Secretion is increased in response to wounding and inflammation (PubMed:25664854). Secretion is increased upon T-cell activation (PubMed:17008549). Neutrophil adhesion to endothelial cells stimulates secretion via gelatinase granules, but foreign particle phagocytosis has no effect (PubMed:10772777). Colocalizes with actin fibers at phagocytic cups (By similarity). Displays calcium-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemisry)

(At 1/200 staining Mouse brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(Staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of ANXA1 (Phospho-Tyr21) using Jurkat whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-ANXA1 antibody

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca (2+) and can bind up to eight Ca (2+) ions (By similarity). Displays Ca (2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca (2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 4502101
• NCBI GeneID: 301
• NCBI Accession #: NP_000691.1
• NCBI GenBank Nucleotide #: NM_000700.1
• UniProt Accession #: P04083
• Molecular Weight: 38,714 Da
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Official Synonym Symbols: ANX1; LPC1
• NCBI Protein Information: annexin A1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein
• UniProt Protein Name: Annexin A1
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1; LPC1
• UniProt Entry Name: ANXA1_HUMAN