WB (Western Blot)
(Western blot analysis of ANO1 using Jurkat whole lysates.)
Rabbit ANO1 Polyclonal Antibody | anti-ANO1 antibody
ANO1 Antibody
Gene Names
ANO1; DOG1; TAOS2; ORAOV2; TMEM16A
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ANO1, Antibody; ANO1 Antibody; ANO 1; ANO1; ANO1_HUMAN; Anoctamin 1; Anoctamin 1 calcium activated chloride channel; Anoctamin-1; Anoctamin1; Ca2+ activated Cl-channel; Calcium Activated Chloride Channel; Discovered on gastrointestinal stromal tumors protein 1; DOG 1; DOG1; FLJ10261; Membrane protein; Oral cancer overexpressed 2; Oral cancer overexpressed protein 2; ORAOV 2; ORAOV2; TAOS 2; TAOS2; TMEM 16A; TMEM16A; Transmembrane protein 16A (eight membrane spanning domains); Transmembrane protein 16A; Tumor amplified and overexpressed sequence 2; Tumor-amplified and overexpressed sequence 2; anti-ANO1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ANO1 antibody detects endogenous levels of total ANO1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
986
Applicable Applications for anti-ANO1 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human ANO1
Subcellular Location
Cell Membrane. Cytoplasm.
Tissue Specificity
Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles.
Predicted Cross Reactivity
Horse, Dog
Similarity
Horse (85%), Dog (85%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of ANO1 using Jurkat whole lysates.)
WB (Western Blot)
(Western blot analysis of ANO1 using Jurkat whole lysates.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using ANO1 Antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using ANO1 Antibody. Lane 1 was treated with the blocking peptide.)
Related Product Information for anti-ANO1 antibody
Function: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.
Subunit Structure: Homodimer (PubMed:21056985). Interacts with CFTR (PubMed:22178883). Interacts with TRPV4 (By similarity).
Similarity: The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. Belongs to the anoctamin family.
Subunit Structure: Homodimer (PubMed:21056985). Interacts with CFTR (PubMed:22178883). Interacts with TRPV4 (By similarity).
Similarity: The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. Belongs to the anoctamin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 90 kDa
Predicted: 115 kDa
Predicted: 115 kDa
NCBI Official Full Name
anoctamin-1
NCBI Official Synonym Full Names
anoctamin 1
NCBI Official Symbol
ANO1
NCBI Official Synonym Symbols
DOG1; TAOS2; ORAOV2; TMEM16A
NCBI Protein Information
anoctamin-1
UniProt Protein Name
Anoctamin-1
UniProt Gene Name
ANO1
UniProt Synonym Gene Names
DOG1; ORAOV2; TAOS2; TMEM16A
Similar Products
Product Notes
The ANO1 ano1 (Catalog #AAA327322) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANO1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ANO1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ANO1 ano1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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