WB (Western Blot)
(All lanes : Anti-ANO10 Antibody (C-Term) at 1:1000-2000 dilutionLane 1: Y79 whole cell lysateLane 2: 293T/17 whole cell lysateLane 3: human heart lysateLane 4: mouse brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 76 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse ANO10 Polyclonal Antibody | anti-ANO10 antibody
ANO10 Antibody (C-Term)
Gene Names
ANO10; SCAR10; TMEM16K
Reactivity
Human, Mouse
Applications
Western Blot
Synonyms
ANO10, Antibody; ANO10 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Anoctamin-10; Transmembrane protein 16K; ANO10; TMEM16K; anti-ANO10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This ANO10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 620-653 amino acids from human ANO10.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
627
Applicable Applications for anti-ANO10 antibody
WB (Western Blot)
Function
Does not exhibit calcium-activated chloride channel (CaCC) activity. Can inhibit the activity of ANO1.
Cellular Location
Cell membrane; Multi-pass membrane protein. Note=Shows predominantly an intracellular localization with a weak expression in the cell membrane
Tissue Location
Highly expressed in the brain. Intermediate levels in the retina and heart and low levels in the placenta, liver, lung, duodenum, kidney, testis and spleen. In brain areas, highest expression in the frontal and occipital cortices and in the cerebellum. Lower expression in the fetal brain than in the adult brain.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-ANO10 Antibody (C-Term) at 1:1000-2000 dilutionLane 1: Y79 whole cell lysateLane 2: 293T/17 whole cell lysateLane 3: human heart lysateLane 4: mouse brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 76 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-ANO10 Antibody (C-Term) at 1:1000-2000 dilutionLane 1: Y79 whole cell lysateLane 2: 293T/17 whole cell lysateLane 3: human heart lysateLane 4: mouse brain lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 76 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Product Categories/Family for anti-ANO10 antibody
References
Ota T.,et al.Nat. Genet. 36:40-45(2004). Muzny D.M.,et al.Nature 440:1194-1198(2006). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Schreiber R.,et al.J. Biol. Chem. 285:7838-7845(2010). Duran C.,et al.Acta Pharmacol. Sin. 32:685-692(2011).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76329
NCBI Official Full Name
anoctamin-10 isoform 2
NCBI Official Synonym Full Names
anoctamin 10
NCBI Official Symbol
ANO10
NCBI Official Synonym Symbols
SCAR10; TMEM16K
NCBI Protein Information
anoctamin-10
UniProt Protein Name
Anoctamin-10
UniProt Gene Name
ANO10
UniProt Synonym Gene Names
TMEM16K
UniProt Entry Name
ANO10_HUMAN
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Product Notes
The ANO10 ano10 (Catalog #AAA290571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANO10 Antibody (C-Term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ANO10 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ANO10 ano10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANO10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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