Rabbit anti-Human APEX1 Polyclonal Antibody | anti-APEX1 antibody

APEX1 Polyclonal Antibody

Cat. Num. AAA37278

• Gene Names: APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purification
• Synonyms: APEX1, Antibody; APEX1 Polyclonal Antibody; APEX1; APE; APE1; APEN; APEX; APX; HAP1; REF1; anti-APEX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 318

• Applicable Applications for anti-APEX1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human APEX1
• Calculated Molecular Weight: 36kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IF (Immunofluorescence)

(Immunofluorescence analysis of A549 cells using APEX1 antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using APEX1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human liver injury using APEX1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using APEX1 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-APEX1 antibody

Ape1 (Apurinic/Apyrimidic eEndonuclease 1), also known as Ref1 (Redox effector factor 1), is a multifunctional protein with several biological activities. These include roles in DNA repair and in the cellular response to oxidative stress. Ape1 initiates the repair of abasic sites and is essential for the base excision repair (BER) pathway (1). Repair activities of Ape1 are stimulated by interaction with XRCC1 (2), another essential protein in BER. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state but can also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation (3). Increased expression of Ape1 is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors (4). Ape1 has been shown to stimulate DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-kappaB, PAX, HIF-1, HLF and p53 (4). Mutation of the Ape1 gene has also been associated with amyotrophic lateral sclerosis (ALS) (5,6).

Product Categories/Family for anti-APEX1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 113984
• NCBI GeneID: 328
• UniProt Accession #: P27695; Q969L5; Q99775
• Molecular Weight: 318
• NCBI Official Full Name: DNA-(apurinic or apyrimidinic site) lyase
• NCBI Official Synonym Full Names: APEX nuclease (multifunctional DNA repair enzyme) 1
• NCBI Official Symbol: APEX1
• NCBI Official Synonym Symbols: APE; APX; APE1; APEN; APEX; HAP1; REF1
• NCBI Protein Information: DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; protein REF-1; redox factor-1; AP endonuclease class I; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic)
• UniProt Protein Name: DNA-(apurinic or apyrimidinic site) lyase
• UniProt Gene Name: APEX1
• UniProt Synonym Gene Names: APE; APE1; APEX; APX; HAP1; REF1; APEN; AP endonuclease 1; APE-1
• UniProt Entry Name: APEX1_HUMAN