Rabbit anti-Human APOB Polyclonal Antibody | anti-APOB antibody

APOB Rabbit pAb

Cat. Num. AAA283293

• Reactivity: Human
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity purification
• Synonyms: APOB, Antibody; APOB Rabbit pAb; FLDB; FCHL2; LDLCQ4; apoB-48; apoB-100; anti-APOB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
• Sequence: LHFSNVFRSVMAPFTMTIDAHTNGNGKLALWGEHTGQLYSKFLLKAEPLAFTFSHDYKGSTSHHLVSRKSISAALEHKVSALLTPAEQTGTWKLKTQFNNNEYSQDLDAYNTKDKIGVELTGRTLADLTLLDSPIKVPLLLSEPINIIDALEMRDAVEKPQEFTIVAFVKYDKNQDVHSINLPFFETLQEYFERNRQTIIVVLENVQRNLKHINIDQFVRKYRAALGKLPQQANDYLNSFNWERQVSHAKEKLTALTKKYRITENDIQIALDDAKINFNEKLSQLQTYMIQFDQYIKDSYD

• Applicable Applications for anti-APOB antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Cross Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1890-2190 of human APOB (NP_000375.3).
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of Hep G2 cells using APOB Rabbit pAb(AAA283293) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using APOB Rabbit pAb (AAA283293) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemisry)

(Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using APOB Rabbit pAb (AAA283293) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohiostchemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using APOB Rabbit pAb (AAA283293) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot)

(Western blot analysis of lysates from Mouse plasma using APOB Rabbit pAb (AAA283293) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time:5s.)

Related Product Information for anti-APOB antibody

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL), and is the ligand for the LDL receptor. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Product Categories/Family for anti-APOB antibody

Cancer; Signal Transduction; Endocrine Metabolism; Lipid Metabolism; Cholesterol Metabolism; Cardiovascular; Heart; Lipids; Cardiovascular diseases; Heart disease; Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 28780
• NCBI GeneID: 338
• UniProt Accession #: P04114; O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8
• Molecular Weight: Calculated MW: 516kDa; Observed MW: 250kDa/520kDa
• UniProt Protein Name: Apolipoprotein B-100
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_HUMAN