FCM/FACS (Flow Cytometry)
(APOBEC3F Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human APOBEC3F Polyclonal Antibody | anti-APOBEC3F antibody
APOBEC3F Antibody (N-term)
Gene Names
APOBEC3F; A3F; KA6; ARP8; BK150C2.4.MRNA
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
APOBEC3F, Antibody; APOBEC3F Antibody (N-term); DNA dC->dU-editing enzyme APOBEC-3F; 354-; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; A3F; APOBEC3F; anti-APOBEC3F antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This APOBEC3F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of human APOBEC3F.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
22-48
Sequence Length
101
Applicable Applications for anti-APOBEC3F antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(APOBEC3F Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(APOBEC3F Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with APOBEC3F Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with APOBEC3F Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of APOBEC3F Antibody (N-term) in K562 cell line lysates (35ug/lane). APOBEC3F (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of APOBEC3F Antibody (N-term) in K562 cell line lysates (35ug/lane). APOBEC3F (arrow) was detected using the purified Pab.)
Related Product Information for anti-APOBEC3F antibody
This protein is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control.
Product Categories/Family for anti-APOBEC3F antibody
References
Khatua,A.K., et.al., Virology 400 (1), 68-75 (2010)
Koning,F.A., et.al., J. Virol. 83 (18), 9474-9485 (2009)
Koning,F.A., et.al., J. Virol. 83 (18), 9474-9485 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45020
NCBI Official Full Name
DNA dC->dU-editing enzyme APOBEC-3F isoform b
NCBI Official Synonym Full Names
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F
NCBI Official Symbol
APOBEC3F
NCBI Official Synonym Symbols
A3F; KA6; ARP8; BK150C2.4.MRNA
NCBI Protein Information
DNA dC->dU-editing enzyme APOBEC-3F
UniProt Protein Name
DNA dC->dU-editing enzyme APOBEC-3F
UniProt Gene Name
APOBEC3F
UniProt Synonym Gene Names
A3F
UniProt Entry Name
ABC3F_HUMAN
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Product Notes
The APOBEC3F apobec3f (Catalog #AAA289117) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 22-48. The APOBEC3F Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOBEC3F can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the APOBEC3F apobec3f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOBEC3F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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