IHC (Immunohistochemisry)
(Apolipoprotein E was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Rabbit Apolipoprotein E Polyclonal Antibody | anti-APOE antibody
Anti-Apolipoprotein E Antibody
Gene Names
Apoe; Apo-E; AI255918
Reactivity
Mouse, RatNo cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Apolipoprotein E, Antibody; Anti-Apolipoprotein E Antibody; AD2; Apo E; APOE; Apo-E; Apolipoprotein E; Apolipoprotein E3; LPG; P08226; apolipoprotein E; anti-APOE antibody
Host
Rabbit
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
311
Applicable Applications for anti-APOE antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Apolipoprotein E was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemisry)
(Apolipoprotein E was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Apolipoprotein E was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Apolipoprotein E was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of Apolipoprotein E expression in mouse spleen extract (lane 1) and mouse kidney extract (lane 2). Apolipoprotein E at 36KD was detected using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of Apolipoprotein E expression in mouse spleen extract (lane 1) and mouse kidney extract (lane 2). Apolipoprotein E at 36KD was detected using rabbit anti- Apolipoprotein E Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-APOE antibody
Rabbit IgG polyclonal antibody for Apolipoprotein E (APOE) detection.
Background: APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Background: APOE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
References
1. APOE epsilon variants increase risk of warfarin-related intracerebral hemorrhage.Falcone GJ, et al. Neurology, 2014 Sep 23.
2. Calabuig-Navarro MV, et al. Apolipoprotein E genotype has a modest impact on the postprandial plasma response to meals of varying fat composition in healthy men in a randomized controlled trial. J Nutr, 2014 Nov.
2. Calabuig-Navarro MV, et al. Apolipoprotein E genotype has a modest impact on the postprandial plasma response to meals of varying fat composition in healthy men in a randomized controlled trial. J Nutr, 2014 Nov.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,867 Da
NCBI Official Full Name
apolipoprotein E
NCBI Official Synonym Full Names
apolipoprotein E
NCBI Official Symbol
Apoe
NCBI Official Synonym Symbols
Apo-E; AI255918
NCBI Protein Information
apolipoprotein E
UniProt Protein Name
Apolipoprotein E
UniProt Gene Name
Apoe
UniProt Synonym Gene Names
Apo-E
UniProt Entry Name
APOE_MOUSE
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Product Notes
The APOE apoe (Catalog #AAA46637) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Apolipoprotein E Antibody reacts with Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein E can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the APOE apoe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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