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IHC (Immunohistochemistry)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, IHC(F)IHC(F): Rat Cardiac Muscle Tissue)
Rabbit Apoptosis inhibitor 5 Polyclonal Antibody | anti-API5 antibody
Anti-Apoptosis inhibitor 5 antibody
Gene Names
API5; AAC11; AAC-11
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Synonyms
Apoptosis inhibitor 5, Antibody; Anti-Apoptosis inhibitor 5 antibody; Apoptosis inhibitor 5(API-5); apoptosis inhibitor 5; AAC 11 antibody; AAC-11 antibody; AAC11 antibody; Antiapoptosis clone 11 protein antibody; API 5 antibody; API-5 antibody; API5 antibody; API5 like 1 antibody; API5_HUMAN antibody; API5L1 antibody; Apoptosis inhibitor 5 antibody; Cell migration inducing gene 8 protein antibody; Cell migration-inducing gene 8 protein antibody; Fibroblast growth factor 2 interacting factor 2 antibody; Fibroblast growth factor 2-interacting factor antibody; FIF antibody; MIG 8 antibody; MIG8 antibody; Migration inducing protein MIG8 antibody; Protein XAGL antibody; anti-API5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
524
Applicable Applications for anti-API5 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
Application Notes
Application details contain suggested dilutions. End user should optimize the final working concentrations.
Immunocytochemistry , 0.5-1ug/ml, Human, Mouse
Immunohistochemistry(Frozen Section), Concentration: 0.5-1ug/ml; Tested Species: Rat; Predicted Species: Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat; Predicted Species: Mouse
Tested Species: In-house tested speceis with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions hsould be determined by end users.
Immunocytochemistry , 0.5-1ug/ml, Human, Mouse
Immunohistochemistry(Frozen Section), Concentration: 0.5-1ug/ml; Tested Species: Rat; Predicted Species: Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat; Predicted Species: Mouse
Tested Species: In-house tested speceis with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions hsould be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5 (487-504aa KYSSNLGNFNYERSLQGK) (b), different from the related mouse sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection System
Preparation and Storage
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemistry)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, IHC(F)IHC(F): Rat Cardiac Muscle Tissue)
IHC (Immunohistochemistry)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, IHC(F)IHC(F): Rat Cardiac Muscle Tissue)
ICC (Immunocytochemistry)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, ICCICC: HELA Cell)
ICC (Immunocytochemistry)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, ICCICC: HELA Cell)
IHC (Immunohistochemistry)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemistry)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, IHC(P)IHC(P): Rat Brain Tissue)
WB (Western Blot)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, Western blottingLane 1: Rat Cardiac Muscle Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Testis Tissue LysateLane 4: Rat Placenta Tissue LysateLane 5: MCF-7 Cell LysateLane 6: HELA Cell LysateLane 7: CEM Cell LysateLane 8: SMMC Cell LysateLane 9: COLO320 Cell Lysate)
WB (Western Blot)
(Anti-Apoptosis inhibitor 5 antibody, AAA11519, Western blottingLane 1: Rat Cardiac Muscle Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Testis Tissue LysateLane 4: Rat Placenta Tissue LysateLane 5: MCF-7 Cell LysateLane 6: HELA Cell LysateLane 7: CEM Cell LysateLane 8: SMMC Cell LysateLane 9: COLO320 Cell Lysate)
Related Product Information for anti-API5 antibody
Background: Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5(API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Apoptosis inhibitor 5
NCBI Official Synonym Full Names
apoptosis inhibitor 5
NCBI Official Symbol
API5
NCBI Official Synonym Symbols
AAC11; AAC-11
NCBI Protein Information
apoptosis inhibitor 5; FIF; antiapoptosis clone 11 protein; migration-inducing protein MIG8; cell migration-inducing gene 8 protein; fibroblast growth factor 2-interacting factor 2
UniProt Protein Name
Apoptosis inhibitor 5
UniProt Gene Name
API5
UniProt Synonym Gene Names
API-5; AAC-11; FIF
UniProt Entry Name
API5_HUMAN
Similar Products
Product Notes
The API5 api5 (Catalog #AAA11519) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Apoptosis inhibitor 5 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Apoptosis inhibitor 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC. Application details contain suggested dilutions. End user should optimize the final working concentrations. Immunocytochemistry, 0.5-1ug/ml, Human, Mouse Immunohistochemistry(Frozen Section), Concentration: 0.5-1ug/ml; Tested Species: Rat; Predicted Species: Human, Mouse Immunohistochemistry(Paraffin-embedded Section), Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Rat; Predicted Species: Mouse Tested Species: In-house tested speceis with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions hsould be determined by end users. Researchers should empirically determine the suitability of the API5 api5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apoptosis inhibitor 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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