ICC (Immunocytochemistry)
(Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/mL.)
Rabbit ARF-BP1 Polyclonal Antibody | anti-ARF-BP1 antibody
ARF-BP1 Antibody
Gene Names
HUWE1; MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column
Synonyms
ARF-BP1, Antibody; ARF-BP1 Antibody; ARF binding protein 1; HECT, UBA and WWE domain containing 1; HUWE1; Mcl-1 ubiquitin ligase E3; Mule; anti-ARF-BP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
4374
Applicable Applications for anti-ARF-BP1 antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
Raised against a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.
Target Name
ARF-BP1
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ICC (Immunocytochemistry)
(Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/mL.)
WB (Western Blot)
(Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/mL.)
WB (Western Blot)
(Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/mL.)
Related Product Information for anti-ARF-BP1 antibody
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
Product Categories/Family for anti-ARF-BP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
481,018 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase HUWE1
NCBI Official Synonym Full Names
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
NCBI Official Symbol
HUWE1
NCBI Official Synonym Symbols
MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
NCBI Protein Information
E3 ubiquitin-protein ligase HUWE1
UniProt Protein Name
E3 ubiquitin-protein ligase HUWE1
UniProt Gene Name
HUWE1
UniProt Synonym Gene Names
KIAA0312; KIAA1578; UREB1; ARF-BP1; HectH9; LASU1; Mule; URE-B1; URE-binding protein 1
UniProt Entry Name
HUWE1_HUMAN
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Product Notes
The ARF-BP1 huwe1 (Catalog #AAA303172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARF-BP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARF-BP1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ARF-BP1 huwe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARF-BP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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