IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cell using ARHGDIA antibody. Blue: DAPI for nuclear staining.)
Rabbit ARHGDIA Polyclonal Antibody | anti-ARHGDIA antibody
ARHGDIA Antibody
Gene Names
ARHGDIA; GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Synonyms
ARHGDIA, Antibody; ARHGDIA Antibody; ARHGDIA; GDIA1; MGC117248; RHOGDI; RHOGDI-1; anti-ARHGDIA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
1mg/mL (varies by lot)
Sequence Length
204
Applicable Applications for anti-ARHGDIA antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant protein of human ARHGDIA
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-292544 / sc-360 / sc-359
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cell using ARHGDIA antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cell using ARHGDIA antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ARHGDIA antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ARHGDIA antibody.)
Related Product Information for anti-ARHGDIA antibody
Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization (Reviewed in 1, 2). The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI (3). Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated signaling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,233 Da
NCBI Official Full Name
ARHGDIA
NCBI Official Synonym Full Names
Rho GDP dissociation inhibitor (GDI) alpha
NCBI Official Symbol
ARHGDIA
NCBI Official Synonym Symbols
GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
NCBI Protein Information
rho GDP-dissociation inhibitor 1; GDP-dissociation inhibitor, aplysia RAS-related 1; epididymis secretory sperm binding protein Li 47e
UniProt Protein Name
Rho GDP-dissociation inhibitor 1
UniProt Gene Name
ARHGDIA
UniProt Synonym Gene Names
GDIA1; Rho GDI 1
UniProt Entry Name
GDIR1_HUMAN
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Product Notes
The ARHGDIA arhgdia (Catalog #AAA169168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGDIA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGDIA can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ARHGDIA arhgdia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGDIA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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