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product-image-AAA211294_IP13.jpg IP (Immunoprecipitation) (Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody AAA211294 (lot AAA211294-2) used for IP at 6 ug per reaction. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody AAA211294-1. For blotting immunoprecipitated ARID1B, AAA211294 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

Rabbit anti-Human ARID1B Polyclonal Antibody | anti-ARID1B antibody

Rabbit anti-ARID1B Antibody, Affinity Purified

Gene Names
ARID1B; OSA2; 6A3-5; DAN15; MRD12; P250R; BRIGHT; BAF250B; ELD/OSA1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
ARID1B, Antibody; Rabbit anti-ARID1B Antibody, Affinity Purified; CSS1; MRD12; Osa homolog 2; P250R; hOsa2; ELD/OSA1; ELD (eyelid)/OSA protein; DAN15; OSA2; BRG1-binding protein hELD/OSA1; BRG1-binding protein ELD/OSA1; BRG1-associated factor 250b; BAF250B; AT-rich interactive domain-containing protein 1B; AT rich interactive domain 1B (SWI1-like); ARID domain-containing protein 1B; 6A3-5; BRIGHT; AT-rich interaction domain 1B; anti-ARID1B antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-ARID1B antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 1950 and 2000
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

IP (Immunoprecipitation)

(Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody AAA211294 (lot AAA211294-2) used for IP at 6 ug per reaction. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody AAA211294-1. For blotting immunoprecipitated ARID1B, AAA211294 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA211294_IP13.jpg IP (Immunoprecipitation) (Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody AAA211294 (lot AAA211294-2) used for IP at 6 ug per reaction. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody AAA211294-1. For blotting immunoprecipitated ARID1B, AAA211294 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohistochemistry)

(Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID1B (Cat. No. AAA211294 Lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA211294_IHC15.jpg IHC (Immunohistochemistry) (Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID1B (Cat. No. AAA211294 Lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-ARID1B antibody
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID1B is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. ARID1B is encoded by a gene independent from ARID1A and exhibits functionally distinct characteristics.
Product Categories/Family for anti-ARID1B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
236,123 Da
NCBI Official Full Name
AT-rich interactive domain-containing protein 1B isoform 1
NCBI Official Synonym Full Names
AT rich interactive domain 1B (SWI1-like)
NCBI Official Symbol
ARID1B
NCBI Official Synonym Symbols
OSA2; 6A3-5; DAN15; MRD12; P250R; BRIGHT; BAF250B; ELD/OSA1
NCBI Protein Information
AT-rich interactive domain-containing protein 1B; ELD (eyelid)/OSA protein; BRG1-associated factor 250b; BRG1-binding protein ELD/OSA1; ARID domain-containing protein 1B
UniProt Protein Name
AT-rich interactive domain-containing protein 1B
UniProt Gene Name
ARID1B
UniProt Synonym Gene Names
BAF250B; DAN15; KIAA1235; OSA2; ARID domain-containing protein 1B; BAF250B; hOsa2
UniProt Entry Name
ARI1B_HUMAN

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Product Notes

The ARID1B arid1b (Catalog #AAA211294) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ARID1B Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARID1B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the ARID1B arid1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARID1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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