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product-image-AAA212014_WB13.jpg WB (Western Blot) (Detection of human and mouse Arpc1b by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Arpc1b antibody AAA212014 (lot AAA212014-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

Rabbit anti-Human, Mouse Arpc1b Polyclonal Antibody | anti-Arpc1b antibody

Rabbit anti-Arpc1b Antibody, Affinity Purified

Gene Names
ARPC1B; ARC41; p40-ARC; p41-ARC
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Arpc1b, Antibody; Rabbit anti-Arpc1b Antibody, Affinity Purified; p41-ARC; PLTEID; p40-ARC; ARP2/3 protein complex subunit p41; arp2/3 complex 41 kDa subunit; ARC41; actin-related protein 2/3 complex subunit 1B; actin related protein 2/3 complex, subunit 1B, 41kDa; Actin-related protein 2/3 complex subunit 1B; actin related protein 2/3 complex subunit 1B; anti-Arpc1b antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-Arpc1b antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 322 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse Arpc1b by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Arpc1b antibody AAA212014 (lot AAA212014-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA212014_WB13.jpg WB (Western Blot) (Detection of human and mouse Arpc1b by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Arpc1b antibody AAA212014 (lot AAA212014-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human Arpc1b by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody AAA212014 (lot AAA212014-3) used for IP at 6 ug per reaction. Arpc1b was also immunoprecipitated by a previous lot of this antibody (lot AAA212014-2) and rabbit anti-Arpc1b antibody For blotting immunoprecipitated Arpc1b, AAA212014 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA212014_IP15.jpg IP (Immunoprecipitation) (Detection of human Arpc1b by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Arpc1b antibody AAA212014 (lot AAA212014-3) used for IP at 6 ug per reaction. Arpc1b was also immunoprecipitated by a previous lot of this antibody (lot AAA212014-2) and rabbit anti-Arpc1b antibody For blotting immunoprecipitated Arpc1b, AAA212014 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-Arpc1b antibody
Arpc1b (actin-related protein 2/3 complex subunit 1B) functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Recently Arpc1b has been found to localize at centrosomes and function in centrosome homeostasis.
Product Categories/Family for anti-Arpc1b antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,950 Da
NCBI Official Full Name
actin-related protein 2/3 complex subunit 1B
NCBI Official Synonym Full Names
actin related protein 2/3 complex, subunit 1B, 41kDa
NCBI Official Symbol
ARPC1B
NCBI Official Synonym Symbols
ARC41; p40-ARC; p41-ARC
NCBI Protein Information
actin-related protein 2/3 complex subunit 1B; arp2/3 complex 41 kDa subunit; ARP2/3 protein complex subunit p41
UniProt Protein Name
Actin-related protein 2/3 complex subunit 1B
UniProt Gene Name
ARPC1B
UniProt Synonym Gene Names
ARC41
UniProt Entry Name
ARC1B_HUMAN

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Product Notes

The Arpc1b arpc1b (Catalog #AAA212014) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Arpc1b Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Arpc1b can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Arpc1b arpc1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arpc1b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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