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IHC (Immunohistochemistry)
(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit ASAP2 Polyclonal Antibody | anti-ASAP2 antibody
Phospho-ASAP2 (Ser701) Antibody
Gene Names
ASAP2; PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
ASAP2, Antibody; Phospho-ASAP2 (Ser701) Antibody; AMAP 2; AMAP2; ANK repeat and pHdomain-containing protein 2; Arf-GAP with SH3 domain; ArfGAP with SH3 domain, ankyrin repeat and pHdomain 2; ASAP2; ASAP2_HUMAN; centaurin, beta 3; CENTB3; DDEF 2; DDEF2; Development and differentiation enhancing factor 2; Development and differentiation-enhancing factor 2; FLJ42910; KIAA0400; PAG 3; PAG3; Pap alpha; PAP; Paxillin associated protein with ARFGAP activity 3; Paxillin-associated protein with ARF GAP activity 3; Pyk2 C terminus associated protein; Pyk2 C-terminus-associated protein; SHAG 1; SHAG1; anti-ASAP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-ASAP2 (Ser701) Antibody detects endogenous levels of ASAP2 only when phosphorylated at Ser701.
Tissue Specificity: Detected in heart, brain, placenta, kidney, monocytes and pancreas.
Tissue Specificity: Detected in heart, brain, placenta, kidney, monocytes and pancreas.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ASAP2 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ASAP2 around the phosphorylation site of Ser701.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated on tyrosine residues by SRC and PTK2B.
Subunit Structure
Binds PXN, ARF1, ARF5, ARF6, PTK2B and SRC.
Similarity
The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.
Subcellular Location
Cytoplasm. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein. Cell membrane>Peripheral membrane protein.
Note: Colocalizes with F-actin and ARF6 in phagocytic cups.
Note: Colocalizes with F-actin and ARF6 in phagocytic cups.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of DDEF2 (Phospho-Ser701) using EGF treated RAW264.7 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of DDEF2 (Phospho-Ser701) using EGF treated RAW264.7 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-ASAP2 antibody
Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
106,982 Da
NCBI Official Full Name
arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform b
NCBI Official Synonym Full Names
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
NCBI Official Symbol
ASAP2
NCBI Official Synonym Symbols
PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
NCBI Protein Information
arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2; PYK2 C terminus-associated protein; centaurin, beta 3; development and differentiation enhancing factor 2; development and differentiation-enhancing factor 2; paxillin-associated prot
UniProt Protein Name
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
UniProt Gene Name
ASAP2
UniProt Synonym Gene Names
DDEF2; KIAA0400; PAG3; PAP
UniProt Entry Name
ASAP2_HUMAN
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Product Notes
The ASAP2 asap2 (Catalog #AAA329864) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ASAP2 (Ser701) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's ASAP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ASAP2 asap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASAP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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