ICC (Immunocytochemistry)
(Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University))
Rabbit Asc Polyclonal Antibody | anti-Asc antibody
Asc (Tyr-144), phospho-specific Antibody
Gene Names
Pycard; Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
Asc, Antibody; Asc (Tyr-144), phospho-specific Antibody; Asc (Tyr-144), phospho-specific; Asc; anti-Asc antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to unphosphorylated Asc (Tyr-144) peptide before affinity purification using phospho-Asc (Tyr-144) peptide (without carrier). The antibody detects phosphorylated Asc (Tyr-144) in the inflammasome. In J774 macrophage cells primed with LPS and treated with nigericin, the antibody colocalized with an inflammasome marker, caspase-1 inhibitor (FAM-YVAD-FMK). In addition, the antibody detects a 20kDa Asc protein in western blots of J774A.1 mouse macrophages treated with pervanadate.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-Asc antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Asc (Tyr-144) phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-144 in mouse Asc. This peptide sequence is highly conserved in human and rat Asc.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University))
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Asc (Tyr-144) in inflammasomes. Paraformaldehyde fixed J774 cells were primed with LPS and treated with nigericin. Cells were co-labeled with DAPI, a caspase-1 inhibitor (FAM-YVAD-FMK), and anti-Asc (Tyr-144) phosphospecific antibody detected with AlexaFluor 568 secondary. (Image provided by Jordan Yaron, Center for Biosignatures Discovery Automation, Arizona State University))
WB (Western Blot)
(Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.)
WB (Western Blot)
(Western blot analysis of mouse macrophage J774A.1 cells stimulated with pervanadate (1mM for 30 min.), then untreated (-) or treated (+) with alkaline phosphatase. The blot was probed with rabbit polyclonal anti-Asc (Tyr-144) phospho-specific antibody (AP5631) at 1:500.)
Related Product Information for anti-Asc antibody
Host- and pathogen-associated cytoplasmic double-stranded DNA triggers the activation of a NALP3-independent inflammasome, which activates caspase-1, leading to maturation of pro-interleukin-1beta and inflammation. Several studies have isolated AIM2 (absent in melanoma 2) as a candidate cytoplasmic-DNA-sensing protein that contains an N-terminal pyrin domain and C-terminal oligonucleotide binding domain. A screen for transcripts induced by interferon-beta identified AIM2 gene expression. AIM2 protein bound double-stranded DNA, recruited the inflammasome adaptor ASC, and localized to ASC containing speckles. AIM2 and ASC form a pyroptosome, which induces pyroptotic cell death mediated by caspase-1. Asc can be phosphorylated at Tyr-144 in a Syk and JNK-dependent manner. This phosphorylation is critical for Asc speck formation and Caspase-1 activation.
References
Hara, H. et al. (2013) Nature Immunol. 14(12):1247.
Roberts, T.L. et al. (2009). Science. 323(5917):1057.
Bürckstümmer, T. et al. (2009). Nat Immunol. 10(3):266.
Roberts, T.L. et al. (2009). Science. 323(5917):1057.
Bürckstümmer, T. et al. (2009). Nat Immunol. 10(3):266.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,459 Da
NCBI Official Full Name
apoptosis-associated speck-like protein containing a CARD
NCBI Official Synonym Full Names
PYD and CARD domain containing
NCBI Official Symbol
Pycard
NCBI Official Synonym Symbols
Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
NCBI Protein Information
apoptosis-associated speck-like protein containing a CARD
UniProt Protein Name
Apoptosis-associated speck-like protein containing a CARD
UniProt Gene Name
Pycard
UniProt Synonym Gene Names
Asc; mASC
UniProt Entry Name
ASC_MOUSE
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Product Notes
The Asc pycard (Catalog #AAA71570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Asc (Tyr-144), phospho-specific Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Asc can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Asc pycard for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Asc, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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