WB (Western Blot)
(Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2) (Origene Technologies).)
Rabbit ASCL1 Polyclonal Antibody | anti-ASCL1 antibody
ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220)
Gene Names
ASCL1; ASH1; HASH1; MASH1; bHLHa46
Reactivity
Human; Predicted Reactivity: Mouse, Rat, Zebrafish
Applications
ELISA, Western Blot
Synonyms
ASCL1, Antibody; ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220); Achaete-scute homolog 1; ASH-1; hASH1; Class A basic helix-loop-helix protein 46; bHLHa46; ASCL1; ASH1; BHLHA46; HASH1; anti-ASCL1 antibody
Host
Rabbit
Reactivity
Human; Predicted Reactivity: Mouse, Rat, Zebrafish
Clonality
Polyclonal
Isotype
Ig
Specificity
This ASCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ASCL1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
236
Applicable Applications for anti-ASCL1 antibody
ELISA, WB (Western Blot)
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Nucleus.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of anti-Ascl1 Antibody (C-term D220) in Hela cell line lysates (35ug/lane). Ascl1(arrow) was detected using the purified Pab (1:160 dilution).)
WB (Western Blot)
(Western blot analysis of anti-Ascl1 Antibody (C-term D220) in Hela cell line lysates (35ug/lane). Ascl1(arrow) was detected using the purified Pab (1:160 dilution).)
WB (Western Blot)
(All lanes : Anti-Ascl1 (Human C-term) at 1:1000 dilutionLane 1: human brain lysateLane 2: A549 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-Ascl1 (Human C-term) at 1:1000 dilutionLane 1: human brain lysateLane 2: A549 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ASCL1 antibody
Description: ACSL1, alternatively titled Hash1 or Mash1, is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. ACSL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. The protein is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers. The presence of a CAG repeat in the gene suggests it may also play a role in tumor formation.
Background: Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5'-CANNTG- 3'). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons (By similarity).
Background: Transcription factor that controls transcriptional expression of its target genes by binding to the E box (5'-CANNTG- 3'). Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Essential for the generation of olfactory and autonomic neurons (By similarity).
Product Categories/Family for anti-ASCL1 antibody
References
Sriuranpong, V., et al., Mol. Cell. Biol. 22 (9):3129-3139 (2002). Westerman, B.A., et al., Clin. Cancer Res. 8 (4):1082-1086 (2002). Chen, H., et al., Cell Growth Differ. 8 (6):677-686 (1997). Borges, M., et al., Nature 386 (6627):852-855 (1997). Renault, B., et al., Genomics 30 (1):81-83 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25454 Da
NCBI Official Full Name
achaete-scute homolog 1
NCBI Official Synonym Full Names
achaete-scute family bHLH transcription factor 1
NCBI Official Symbol
ASCL1
NCBI Official Synonym Symbols
ASH1; HASH1; MASH1; bHLHa46
NCBI Protein Information
achaete-scute homolog 1
UniProt Protein Name
Achaete-scute homolog 1
UniProt Gene Name
ASCL1
UniProt Synonym Gene Names
ASH1; BHLHA46; HASH1; ASH-1; hASH1; bHLHa46
UniProt Entry Name
ASCL1_HUMAN
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Product Notes
The ASCL1 ascl1 (Catalog #AAA290658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220) reacts with Human; Predicted Reactivity: Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's ASCL1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ASCL1 ascl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASCL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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