IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed paraffin-embedded tissue section of human small venous vessels stained for ASK1 expression using at 1: 2000. ASK1 positive epithelial cells were seen. Hematoxylin-eosin counterstain.)
Rabbit anti-Human ASK1 Polyclonal Antibody | anti-ASK1 antibody
Polyclonal Antibody to ASK1
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Serum
Synonyms
ASK1, Antibody; Polyclonal Antibody to ASK1; MAP3K5; ASK1; MAPKKK5; MEKK5; Apoptosis signal-regulating kinase 1; MEK kinase 5; MAPK/ERK kinase kinase 5; Antibody to ASK1; Anti-ASK1 Antibody; ASK1 Antibody; anti-ASK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
1374
Applicable Applications for anti-ASK1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen Information
A synthetic peptide of ASK1 protein (amino acids 1198- 1216 CHEEQPSNQTVRRPQAVIED) was used as the immunogen for this antibody
Antigen
ASK1
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed paraffin-embedded tissue section of human small venous vessels stained for ASK1 expression using at 1: 2000. ASK1 positive epithelial cells were seen. Hematoxylin-eosin counterstain.)
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed paraffin-embedded tissue section of human small venous vessels stained for ASK1 expression using at 1: 2000. ASK1 positive epithelial cells were seen. Hematoxylin-eosin counterstain.)
IHC (Immunohistochemistry)
(Fig: 1 Formalin-fixed paraffin-embedded tissue section of human reactive tonsil stained for ASK1 expression using at 1: 2000. A and A1. Low and high magnification, respectively. ASK1 expression was seen in the vasculature (endothelial cells). Hematoxylin-eosin counterstain.)
IHC (Immunohistochemistry)
(Fig: 1 Formalin-fixed paraffin-embedded tissue section of human reactive tonsil stained for ASK1 expression using at 1: 2000. A and A1. Low and high magnification, respectively. ASK1 expression was seen in the vasculature (endothelial cells). Hematoxylin-eosin counterstain.)
Related Product Information for anti-ASK1 antibody
Ask1 (apoptosis signal-regulating kinase 1), a serine/threonine kinase, is a member of the MAPKKK family that constitutes the JNK and p38 (MAPK) cascades. Members of this family are activated by physiological and cytotoxic stresses and induce various stress responses including apoptosis. Specifically, Ask1-MAPK cascades have been shown to be involved in endoplasmic reticululum (ER) stress-induced apoptosis and in neuronal cell death. Studies suggest that ER stress-induced apoptosis plays critical roles in the pathogenesis in some neurodegenerative diseases including Alzheimer's, Parkinson's, polyglutamine (polyQ), amyotrophic lateral sclerosis (ALS) and Prion diseases. However, the regulation and function of Ask1 in neuronal cell death remains to be fully elucidated. In addition to playing a role in ER stress-induced apoptosis, Ask1 is also thought to be important in cytokine-induced apoptosis, and may also have functions other than apoptosis including roles in cell survival and differentiation. Structurally, human Ask1 has a serine/threonine kinase domain in the middle of the molecule. The kinase domain is evolutionarily conserved from nematode to human. A threonine residue with the activation loop of the kinases domain (Thr838 of human Ask1) is essential for Ask1 activation. There are two coiled-coil domains, one in the N-terminal and the other in the C-terminal domain. The coiled-coil domain in the C-terminal domain has been shown to be required for homo-oligomerzation.
Product Categories/Family for anti-ASK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
69,839 Da
NCBI Official Full Name
Mitogen-activated protein kinase kinase kinase 5
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol
MAP3K5
NCBI Official Synonym Symbols
ASK1; MEKK5; MAPKKK5
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 5
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 5
UniProt Gene Name
MAP3K5
UniProt Synonym Gene Names
ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5
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Product Notes
The ASK1 map3k5 (Catalog #AAA78298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to ASK1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ASK1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the ASK1 map3k5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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