WB (Western Blot)
(Detection of human and mouse Aspartyl-tRNA Synthetase by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aspartyl-tRNA Synthetase antibody AAA212944 (lot AAA212944-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse Aspartyl-tRNA Synthetase/DARS Polyclonal Antibody | anti-DARS antibody
Rabbit anti-Aspartyl-tRNA Synthetase/DARS Antibody, Affinity Purified
Gene Names
DARS; HBSL; aspRS
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Aspartyl-tRNA Synthetase/DARS, Antibody; Rabbit anti-Aspartyl-tRNA Synthetase/DARS Antibody, Affinity Purified; aspRS; testicular tissue protein Li 192; cell proliferation-inducing gene 40 protein; aspartyl-tRNA synthetase, cytoplasmic; Aspartyl-tRNA synthetase; aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1, cytoplasmic; HBSL; Aspartate--tRNA ligase, cytoplasmic; aspartyl-tRNA synthetase; anti-DARS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DARS antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Aspartyl-tRNA Synthetase by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aspartyl-tRNA Synthetase antibody AAA212944 (lot AAA212944-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse Aspartyl-tRNA Synthetase by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aspartyl-tRNA Synthetase antibody AAA212944 (lot AAA212944-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Aspartyl-tRNA Synthetase by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aspartyl-tRNA Synthetase antibody AAA212944 (lot AAA212944-1) used for IP at 6 ug per reaction. Aspartyl-tRNA Synthetase was also immunoprecipitated by rabbit anti-Aspartyl-tRNA Synthetase antibody BL18508. For blotting immunoprecipitated Aspartyl-tRNA Synthetase, AAA212944 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Aspartyl-tRNA Synthetase by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aspartyl-tRNA Synthetase antibody AAA212944 (lot AAA212944-1) used for IP at 6 ug per reaction. Aspartyl-tRNA Synthetase was also immunoprecipitated by rabbit anti-Aspartyl-tRNA Synthetase antibody BL18508. For blotting immunoprecipitated Aspartyl-tRNA Synthetase, AAA212944 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-DARS antibody
Aspartyl-tRNA Synthetase/DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14868].
Product Categories/Family for anti-DARS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,771 Da
NCBI Official Full Name
aspartate--tRNA ligase, cytoplasmic isoform 1
NCBI Official Synonym Full Names
aspartyl-tRNA synthetase
NCBI Official Symbol
DARS
NCBI Official Synonym Symbols
HBSL; aspRS
NCBI Protein Information
aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing gene 40 protein
UniProt Protein Name
Aspartate--tRNA ligase, cytoplasmic
UniProt Gene Name
DARS
UniProt Synonym Gene Names
AspRS
UniProt Entry Name
SYDC_HUMAN
Similar Products
Product Notes
The DARS dars (Catalog #AAA212944) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Aspartyl-tRNA Synthetase/DARS Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartyl-tRNA Synthetase/DARS can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DARS dars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartyl-tRNA Synthetase/DARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.