Rabbit anti-Human, Mouse ATF6 Polyclonal Antibody | anti-ATF6 antibody

ATF6 Antibody

Cat. Num. AAA10957

• Gene Names: ATF6; ATF6A
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ATF6 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ATF6; Polyclonal Antibody; ATF6 Antibody; ATF6A; Cyclic AMP-dependent transcription factor ATF-6 alpha; Activating transcription factor 6 alpha; cAMP-dependent transcription factor ATF-6 alpha; activating transcription factor 6; anti-ATF6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ATF6 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 670

• Applicable Applications for anti-ATF6 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6.
• Buffer: ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ATF6 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 μg/ml.Green: ATF6 Antibody (3681)Blue: DAPI staining)

IHC (Immunohistchemistry)

(Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibody at 5 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 μg/ml.Green: ATF6 Antibody (3681)Blue: DAPI staining)

IHC (Immunohistochemistry)

(Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 μg/mL.)

ICC (Immunocytochemistry)

(Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 μg/mL.)

WB (Western Blot)

(Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-ATF6 antibody

ATF6 Antibody: Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6α and ATF6β); only ATF6α is recognized by this antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 56786157
• NCBI GeneID: 22926
• NCBI Accession #: NP_031374
• NCBI GenBank Nucleotide #: NM_007348.3
• UniProt Accession #: P18850; O15139; Q5VW62; Q6IPB5; Q9UEC9
• Molecular Weight: 74,585 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-6 alpha
• NCBI Official Synonym Full Names: activating transcription factor 6
• NCBI Official Symbol: ATF6
• NCBI Official Synonym Symbols: ATF6A
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-6 alpha
• UniProt Gene Name: ATF6
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha
• UniProt Entry Name: ATF6A_HUMAN