IHC (Immunohistochemistry)
(Figure 8 Immunohistochemistry Validation of ATF6 in Rat Brain Tissue Immunohistochemical analysis of paraffin-embedded Rat brain tissue using anti-ATF6 antibody (AAA10937) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Rabbit ATF6 Polyclonal Antibody | anti-ATF6 antibody
ATF6 Antibody
Optimal dilutions/concentrations should be determined by the end user.
The immunogen is located within amino acids 30-80 of ATF6
IHC (Immunohistochemistry)
(Figure 8 Immunohistochemistry Validation of ATF6 in Rat Brain Tissue Immunohistochemical analysis of paraffin-embedded Rat brain tissue using anti-ATF6 antibody (AAA10937) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 8 Immunohistochemistry Validation of ATF6 in Rat Brain Tissue Immunohistochemical analysis of paraffin-embedded Rat brain tissue using anti-ATF6 antibody (AAA10937) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 7 Immunohistochemistry Validation of ATF6 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATF6 antibody (AAA10937) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 7 Immunohistochemistry Validation of ATF6 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATF6 antibody (AAA10937) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of ATF6 in Human Testis Cells Immunofluorescent analysis of 4% paraformaldehydefixed human testis labeling ATF6 with AAA10937 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of ATF6 in Human Testis Cells Immunofluorescent analysis of 4% paraformaldehydefixed human testis labeling ATF6 with AAA10937 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of ATF6 In A549 Cells Immunofluorescent analysis of 4% paraformaldehydefixed A549 cells labeling ATF6 with AAA10937 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of ATF6 In A549 Cells Immunofluorescent analysis of 4% paraformaldehydefixed A549 cells labeling ATF6 with AAA10937 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Loading: 15 ug of rat kidney lysate Antibodies: ATF6 (AAA10937), 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Loading: 15 ug of rat kidney lysate Antibodies: ATF6 (AAA10937), 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 WB Validation in Mouse Tissue Loading: 15 ug of mouse tissue lysate Antibodies: ATF6 (AAA10937), 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 WB Validation in Mouse Tissue Loading: 15 ug of mouse tissue lysate Antibodies: ATF6 (AAA10937), 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 WB Validation in Human Cell Lines Loading: 15 ug of human cell lysate Antibodies ATF6 (AAA10937), 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 WB Validation in Human Cell Lines Loading: 15 ug of human cell lysate Antibodies ATF6 (AAA10937), 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. conjugate at 1:10000 dilution.)
WB (Western Blot)
(Loading: 15 ug of human HeLa cell lysate Antibodies ATF6 (150934), 0.5 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Loading: 15 ug of human HeLa cell lysate Antibodies ATF6 (150934), 0.5 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
NCBI and Uniprot Product Information
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Product Notes
The ATF6 atf6 (Catalog #AAA10937) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATF6 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Optimal dilutions for each application to be determined by the researcher. Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the ATF6 atf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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