Rabbit anti-Human ATP5D Polyclonal Antibody | anti-ATP5F1D antibody

Anti-ATP5D Antibody, Rabbit Polyclonal

Cat. Num. AAA255779

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: ATP5D, Antibody; Anti-ATP5D Antibody, Rabbit Polyclonal; ATP5D Antibody, Rabbit PAb, Antigen Affinity Purified; ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit; anti-ATP5F1D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ATP5D
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ATP5F1D antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human ATP5D protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ATP5D (rh ATP5D). ATP5D specific IgG was purified by Human ATP5D affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(ATP5D was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Raji Whole Cell LysateLane C:0.5 mg A549 Whole Cell Lysate2 uL anti-ATP5D rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATP5D rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 13 kDaObserved band size: 13 kDa)

WB (Western Blot)

(Anti-ATP5D rabbit polyclonal antibody at 1:500 dilutionLane A: HEPG2 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: MOLT-4 Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:14 kDa(We are unsure as to the identity of these extra bands.))

IHC (Immunohiostchemistry)

(Immunochemical staining of human ATP5D in human hepatoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry)

(Immunochemical staining of human ATP5D in human lung cancer with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-ATP5F1D antibody

ATP5D is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase consists of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5D gene encodes the delta subunit of the catalytic core.

References

Jordan EM. et al., 1992, Biochim Biophys Acta 1130 (1): 123-6. Yoshida M. et al., 2001, Nat Rev Mol Cell Biol. 2 (9): 669-77. Hochstrasser DF. et al., 1993, Electrophoresis. 13 (12): 992-1001.

NCBI and Uniprot Product Information

• NCBI GI #: 50345991
• NCBI GeneID: 513
• NCBI Accession #: NP_001001975.1
• NCBI GenBank Nucleotide #: NM_001001975.1
• UniProt Accession #: P30049; Q6FG90; D6W5Y3
• Molecular Weight: 17,490 Da
• NCBI Official Full Name: ATP synthase subunit delta, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
• NCBI Official Symbol: ATP5D
• NCBI Protein Information: ATP synthase subunit delta, mitochondrial; F-ATPase delta subunit; mitochondrial ATP synthase, delta subunit; mitochondrial ATP synthase complex delta-subunit precusor
• UniProt Protein Name: ATP synthase subunit delta, mitochondrial
• UniProt Gene Name: ATP5D
• UniProt Entry Name: ATPD_HUMAN