Rabbit ATP5J Polyclonal Antibody | anti-ATP5J antibody

ATP5J Antibody (Center)

Cat. Num. AAA285507

• Gene Names: ATP5J; F6; CF6; ATP5; ATPM; ATP5A
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Monkey
• Applications: Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: ATP5J, Antibody; ATP5J Antibody (Center); ATP synthase-coupling factor 6; mitochondrial; ATPase subunit F6; ATP5J; ATP5A; ATPM; anti-ATP5J antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Monkey
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 28-56
• Sequence Length: 116

• Applicable Applications for anti-ATP5J antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
• Cellular Location: Mitochondrion. Mitochondrion inner membrane.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(ATP5J Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP5J Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

IF (Immunofluorescence)

(Fluorescent confocal image of U251 cell stained with ATP5J Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ATP5J primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ATP5J immunoreactivity is localized to Mitochondria significantly.)

WB (Western Blot)

(ATP5J Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the ATP5J antibody detected the ATP5J protein (arrow).)

WB (Western Blot)

(Western blot analysis of lysate from rat heart tissue lysate, using ATP5J Antibody (Center). AAA285507 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Product Categories/Family for anti-ATP5J antibody

Crown Antibodies; Metabolism; Signal Transduction

References

Osanai, T., et al. Cardiovasc. Res. 81(4):780-787(2009)
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Kumagai, A., et al. Atherosclerosis 200(1):45-50(2008)
Chai, S.B., et al. Circ. J. 71(5):693-697(2007)
Morava, E., et al. Am. J. Med. Genet. A 140(8):863-868(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 51479137
• NCBI GeneID: 522
• NCBI Accession #: NP_001003696.1; NP_001003697.1; NP_001003701.1; NP_001003703.1; NP_001676.2
• NCBI GenBank Nucleotide #: NM_001003696.1
• UniProt Accession #: P18859; J3KQ83
• NCBI Official Full Name: ATP synthase-coupling factor 6, mitochondrial isoform a
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
• NCBI Official Symbol: ATP5J
• NCBI Official Synonym Symbols: F6; CF6; ATP5; ATPM; ATP5A
• NCBI Protein Information: ATP synthase-coupling factor 6, mitochondrial
• UniProt Protein Name: ATP synthase-coupling factor 6, mitochondrial
• UniProt Gene Name: ATP5J
• UniProt Synonym Gene Names: ATP5A; ATPM; ATPase subunit F6
• UniProt Entry Name: ATP5J_HUMAN