Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: ATP1b3 knockout A431 cell lysate; Predicted MW: 32kd Observed MW: 55kd Primary Ab: 5ug/ml Rabbit Anti-Human ATP1b3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) Polyclonal Antibody | anti-ATP1b3 antibody
FITC-linked Antibody to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3)
Gene Names
ATP1B3; CD298; ATPB-3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Antigen-specific Affinity Chromatography
Synonyms
ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3), Antibody; FITC-linked Antibody to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3); anti-ATP1b3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific Affinity Chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence Length
198
Applicable Applications for anti-ATP1b3 antibody
FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Source
Antibody labeling
Label
FITC
Traits
Liquid
Immunogen
Recombinant ATP1b3 (Met61~Phe273) expressed in E.coli.
Cross Reactivity
Human, Rat
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Note: As fluorescence can photobleach when exposed to light, so the antibody must be protected from light.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Note: As fluorescence can photobleach when exposed to light, so the antibody must be protected from light.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: ATP1b3 knockout A431 cell lysate; Predicted MW: 32kd Observed MW: 55kd Primary Ab: 5ug/ml Rabbit Anti-Human ATP1b3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: ATP1b3 knockout A431 cell lysate; Predicted MW: 32kd Observed MW: 55kd Primary Ab: 5ug/ml Rabbit Anti-Human ATP1b3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-ATP1b3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
ATPase, Na+/K+ transporting, beta 3 polypeptide, isoform CRA_f
NCBI Official Synonym Full Names
ATPase, Na+/K+ transporting, beta 3 polypeptide
NCBI Official Symbol
ATP1B3
NCBI Official Synonym Symbols
CD298; ATPB-3
NCBI Protein Information
sodium/potassium-transporting ATPase subunit beta-3; sodium pump subunit beta-3; Na, K-ATPase beta-3 polypeptide; sodium/potassium-dependent ATPase beta-3 subunit; sodium/potassium-dependent ATPase subunit beta-3; sodium/potassium-transporting ATPase beta-3 chain; sodium-potassium ATPase subunit beta 3 (non-catalytic)
UniProt Protein Name
Sodium/potassium-transporting ATPase subunit beta-3
UniProt Gene Name
ATP1B3
UniProt Synonym Gene Names
ATPB-3
UniProt Entry Name
AT1B3_HUMAN
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Product Notes
The ATP1b3 atp1b3 (Catalog #AAA134942) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FITC-linked Antibody to ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ATP1b3 atp1b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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