Rabbit Atrogin-1 Polyclonal Antibody | anti-Fbxo32 antibody

Atrogin-1 Antibody

Cat. Num. AAA71564

• Gene Names: Fbxo32; MAFbx; Gm20361; AI430017; ATROGIN1; 4833442G10Rik
• Reactivity: Human, Rat, Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: Atrogin-1, Antibody; Atrogin-1 Antibody; Atrogin-1; Atrogin pAb; anti-Fbxo32 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody detects a 41kDa* protein corresponding to the apparent molecular mass of Atrogin on SDS-PAGE immunoblots of mouse muscle tissue lysates. Similar results were seen in C2C12 cell lysate and the band observed in western blot is specifically blocked by Atrogin-1 peptide (AX2045).
• Form/Format: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

• Applicable Applications for anti-Fbxo32 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide (coupled to carrier protein) corresponding to amino acids 23 to 35 in mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32).
• Preparation and Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

IHC (Immunohiostchemistry)

(Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 (AAA71564) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).)

WB (Western Blot)

(Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diagphram tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (AAA71564; lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide (AX2045).)

Related Product Information for anti-Fbxo32 antibody

Atrogin-1/Muscle Atrophy F-box (MAFbx) is an E3 ubiquitin ligase that mediates proteolysis events that occur during muscle atrophy. This ATP-dependent ubiquitin-mediated proteolysis occurs in response to a variety of catabolic states in muscle. Atrogin is expressed in heart and skeletal muscle, and is upregulated during muscle atrophy. In addition, Atrogin expression increases in C2C12 myotubes after stimulation with cytokines. Atrogin is thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Atrogin interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD. Mice deficient in Atrogin are resistant to muscle atrophy.

References

Bodine, S.C. et al. (2001) Science 294: 1704-8.
Dai, K.S. & Liews, C.C. (2001) J Biol. Chem. 276(26):23992.
Leger, B. et al. (2006) J Physiol. 576(3):923.

NCBI and Uniprot Product Information

• NCBI GI #: 13385848
• NCBI GeneID: 67731
• NCBI Accession #: NP_080622.1
• NCBI GenBank Nucleotide #: NM_026346.3
• UniProt Accession #: Q9CPU7
• Molecular Weight: 41 kDa
• NCBI Official Full Name: F-box only protein 32
• NCBI Official Synonym Full Names: F-box protein 32
• NCBI Official Symbol: Fbxo32
• NCBI Official Synonym Symbols: MAFbx; Gm20361; AI430017; ATROGIN1; 4833442G10Rik
• NCBI Protein Information: F-box only protein 32
• UniProt Protein Name: F-box only protein 32
• UniProt Gene Name: Fbxo32
• UniProt Synonym Gene Names: MAFbx
• UniProt Entry Name: FBX32_MOUSE