IHC (Immunohistochemisry)
(Immunofluorescent analysis of HeLa cells using AAA231199 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human AURKA Polyclonal Antibody | anti-AURKA antibody
AURKA Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
AURKA, Antibody; AURKA Antibody; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/thre; AURKA; AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; anti-AURKA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
403
Applicable Applications for anti-AURKA antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Species
Human
Immunogen
Recombinant human Aurora kinase A protein
Conjugate
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-14321 / sc-14318 / sc-25425
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohistochemisry)
(Immunofluorescent analysis of HeLa cells using AAA231199 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of HeLa cells using AAA231199 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using AAA231199 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human ovarian cancer using AAA231199 at dilution of 1:100)
WB (Western Blot)
(Western blotAll lanes :AURKA antibody at 2 ug/mlLane 1 : NIH/3T3 whole cell lysateLane 2 : HT29 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 46 kDaObserved band size: 46,100 kDa)
WB (Western Blot)
(Western blotAll lanes :AURKA antibody at 2 ug/mlLane 1 : NIH/3T3 whole cell lysateLane 2 : HT29 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 46 kDaObserved band size: 46,100 kDa)
Related Product Information for anti-AURKA antibody
Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,809 Da
NCBI Official Full Name
aurora kinase A
NCBI Official Synonym Full Names
aurora kinase A
NCBI Official Symbol
AURKA
NCBI Official Synonym Symbols
AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
NCBI Protein Information
aurora kinase A
UniProt Protein Name
Aurora kinase A
UniProt Gene Name
AURKA
UniProt Synonym Gene Names
AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; ARK-1; Aurora-related kinase 1; hARK1
UniProt Entry Name
AURKA_HUMAN
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Product Notes
The AURKA aurka (Catalog #AAA231199) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AURKA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AURKA can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the AURKA aurka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AURKA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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