IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, using AurB Antibody. The picture on the right is blocked with the synthesized peptide.)
Rabbit AURKB Polyclonal Antibody | anti-AurB antibody
AurB Antibody
Gene Names
AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
Reactivity
Human; Mouse; Rat; Monkey
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
AURKB, Antibody; AurB Antibody; AURKB; AIK2; AIM1; AIRK2; ARK2; STK1; STK12;STK5;Aurora kinase B; Aurora 1; Auroraand IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12; anti-AurB antibody
Host
Rabbit
Reactivity
Human; Mouse; Rat; Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
ARK-2 Polyclonal Antibody detects endogenous levels of ARK-2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-AurB antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized peptide derived from human AurB. AA range: 198-247
Function
Catalytic activity: ATP + a protein = ADP + a phosphoprotein., cofactor: Magnesium., disease: Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.,function: May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis., similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Subcellular Location
Nucleus. Chromosome. Chromosome, centromere. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle . Midbody . Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis (PubMed: 20929775). Colocalized with gamma tubulin in the midbody (PubMed: 17726514). Proper localization of the active, Thr-232-phosphorylated form during metaphase may be dependent upon interaction with SPDYC (PubMed: 20605920). Colocalized with SIRT2 during cytokinesis with the midbody (PubMed: 17726514). Localization (and probably targeting of the CPC) to the inner centromere occurs predominantly in regions with overlapping mitosis-specific histone phosphorylations H3pT3 and H2ApT12.
Expression
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.; [Isoform 3]: Not expressed in normal liver, high expression in metastatic liver.
Preparation and Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, using AurB Antibody. The picture on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells, using AurB Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot)
(Western Blot analysis of COS7 cells using ARK-2 Polyclonal Antibody cells nucleus extracted by Minute Cytoplasmic and Nuclear Fractionation kit.)
WB (Western Blot)
(Western Blot analysis of COS7 cells using ARK-2 Polyclonal Antibody cells nucleus extracted by Minute Cytoplasmic and Nuclear Fractionation kit.)
Related Product Information for anti-AurB antibody
This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Band(KD): 39kD
NCBI Official Full Name
aurora kinase B isoform 2
NCBI Official Synonym Full Names
aurora kinase B
NCBI Official Symbol
AURKB
NCBI Official Synonym Symbols
AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
NCBI Protein Information
aurora kinase B
UniProt Protein Name
Aurora kinase B
UniProt Gene Name
AURKB
UniProt Synonym Gene Names
AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2; Aurora-related kinase 2
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Product Notes
The AurB aurkb (Catalog #AAA267899) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AurB Antibody reacts with Human; Mouse; Rat; Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's AURKB can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AurB aurkb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AURKB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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