WB (Western Blot)
(Aurora B pAb tested by Western blot Nuclear extract of U2OS cells (20 ug per lane) probed with Aurora B polyclonal antibody (1:500 dilution).)
Rabbit Aurora B Polyclonal Antibody | anti-AuroraB antibody
Aurora B antibody (pAb)
Gene Names
AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
Reactivity
Human, Mouse, Wide Range Predicted
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Synonyms
Aurora B, Antibody; Aurora B antibody (pAb); Aurora Kinase B; AurB; ARK2; anti-AuroraB antibody
Host
Rabbit
Reactivity
Human, Mouse, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-AuroraB antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
This Aurora B antibody was raised against a peptide including the C-terminus of human Aurora B.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Aurora B pAb tested by Western blot Nuclear extract of U2OS cells (20 ug per lane) probed with Aurora B polyclonal antibody (1:500 dilution).)
WB (Western Blot)
(Aurora B pAb tested by Western blot Nuclear extract of U2OS cells (20 ug per lane) probed with Aurora B polyclonal antibody (1:500 dilution).)
IF (Immunofluorescence)
(Aurora B pAb tested by immunofluorescence. Top left: HeLa cells stained with Aurora B polyclonal antibody at a 1:200 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.)
IF (Immunofluorescence)
(Aurora B pAb tested by immunofluorescence. Top left: HeLa cells stained with Aurora B polyclonal antibody at a 1:200 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.)
Related Product Information for anti-AuroraB antibody
Short Description: Our Aurora B antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
Background: Aurora B (Aurora Kinase B, AurB, ARK2) is a Ser/Thr kinase that is part of the chromosomal passenger complex that associates with chromosomes and is an important regulator of mitosis and meiosis. Aurora B becomes concentrated at the centromere during the onset of mitosis, but relocates to the spindle midzone during anaphase. Aurora B phosphorylates a number of substrates, including INCENP, CENP-A, and Histone H3. Misregulation of Aurora B activity can lead to chromosome instability and is implicated in a number of cancers.
Background: Aurora B (Aurora Kinase B, AurB, ARK2) is a Ser/Thr kinase that is part of the chromosomal passenger complex that associates with chromosomes and is an important regulator of mitosis and meiosis. Aurora B becomes concentrated at the centromere during the onset of mitosis, but relocates to the spindle midzone during anaphase. Aurora B phosphorylates a number of substrates, including INCENP, CENP-A, and Histone H3. Misregulation of Aurora B activity can lead to chromosome instability and is implicated in a number of cancers.
Product Categories/Family for anti-AuroraB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,467 Da
NCBI Official Full Name
aurora kinase B isoform 1
NCBI Official Synonym Full Names
aurora kinase B
NCBI Official Symbol
AURKB
NCBI Official Synonym Symbols
AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
NCBI Protein Information
aurora kinase B
UniProt Protein Name
Aurora kinase B
UniProt Gene Name
AURKB
UniProt Synonym Gene Names
AIK2; AIM1; AIRK2; ARK2; STK1; STK12; STK5; AIM-1; ARK-2
UniProt Entry Name
AURKB_HUMAN
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Product Notes
The AuroraB aurkb (Catalog #AAA59853) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aurora B antibody (pAb) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Aurora B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the AuroraB aurkb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aurora B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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