IHC (Immunohiostchemistry)
(AXIN1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AXIN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human AXIN1 Polyclonal Antibody | anti-AXIN1 antibody
AXIN1 Antibody (C-term)
Gene Names
AXIN1; AXIN; PPP1R49
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AXIN1, Antibody; AXIN1 Antibody (C-term); Axin-1; Axis inhibition protein 1; hAxin; AXIN1; AXIN; anti-AXIN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AXIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 710-738 amino acids from the C-terminal region of human AXIN1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
710-738
Sequence Length
862
Applicable Applications for anti-AXIN1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(AXIN1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AXIN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(AXIN1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AXIN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(AXIN1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the AXIN1 antibody detected the AXIN1 protein (arrow).)
WB (Western Blot)
(AXIN1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the AXIN1 antibody detected the AXIN1 protein (arrow).)
Related Product Information for anti-AXIN1 antibody
This gene encodes a cytoplasmic protein which contains a
regulation of G-protein signaling (RGS) domain and a dishevelled
and axin (DIX) domain. The encoded protein interacts with
adenomatosis polyposis coli, catenin beta-1, glycogen synthase
kinase 3 beta, protein phosphate 2, and itself. This protein
functions as a negative regulator of the wingless-type MMTV
integration site family, member 1 (WNT) signaling pathway and can
induce apoptosis. The crystal structure of a portion of this
protein, alone and in a complex with other proteins, has been
resolved. Mutations in this gene have been associated with
hepatocellular carcinoma, hepatoblastomas, ovarian endometriod
adenocarcinomas, and medullablastomas. Two transcript variants
encoding distinct isoforms have been identified for this gene.
regulation of G-protein signaling (RGS) domain and a dishevelled
and axin (DIX) domain. The encoded protein interacts with
adenomatosis polyposis coli, catenin beta-1, glycogen synthase
kinase 3 beta, protein phosphate 2, and itself. This protein
functions as a negative regulator of the wingless-type MMTV
integration site family, member 1 (WNT) signaling pathway and can
induce apoptosis. The crystal structure of a portion of this
protein, alone and in a complex with other proteins, has been
resolved. Mutations in this gene have been associated with
hepatocellular carcinoma, hepatoblastomas, ovarian endometriod
adenocarcinomas, and medullablastomas. Two transcript variants
encoding distinct isoforms have been identified for this gene.
Product Categories/Family for anti-AXIN1 antibody
References
Sue Ng, S., et al. Biol. Chem. 391 (2-3), 171-180 (2010) :
Yang, L.H., et al. Mol. Cancer 9, 25 (2010) :
Wooten, E.C., et al. PLoS ONE 5 (1), E8830 (2010) :
Kameoka, M., et al. AIDS Res. Hum. Retroviruses 25(10):1005-1011(2009)
Li, Q., et al. Nat. Cell Biol. 11(9):1128-1134(2009)
Yang, L.H., et al. Mol. Cancer 9, 25 (2010) :
Wooten, E.C., et al. PLoS ONE 5 (1), E8830 (2010) :
Kameoka, M., et al. AIDS Res. Hum. Retroviruses 25(10):1005-1011(2009)
Li, Q., et al. Nat. Cell Biol. 11(9):1128-1134(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95635
NCBI Official Full Name
axin-1 isoform a
NCBI Official Synonym Full Names
axin 1
NCBI Official Symbol
AXIN1
NCBI Official Synonym Symbols
AXIN; PPP1R49
NCBI Protein Information
axin-1
UniProt Protein Name
Axin-1
UniProt Gene Name
AXIN1
UniProt Synonym Gene Names
AXIN; hAxin
UniProt Entry Name
AXIN1_HUMAN
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Product Notes
The AXIN1 axin1 (Catalog #AAA283724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 710-738. The AXIN1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AXIN1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AXIN1 axin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AXIN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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