Goat AXIN1 / Axin-1 Polyclonal Antibody | anti-AXIN1 antibody

Goat Polyclonal to Human AXIN1 / Axin-1

Cat. Num. AAA50903

• Gene Names: AXIN1; AXIN; PPP1R49
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Zebrafish
• Applications: ELISA, Immunohistochemistry, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: AXIN1 / Axin-1, Antibody; Goat Polyclonal to Human AXIN1 / Axin-1; Anti-AXIN1 / Axin-1 Antibody (C-Terminus) IHC-plus; AVPR2; ADHR; AVPR V2; Avp2r; Antidiuretic hormone receptor; DI1; DIR3; NDI; Vasopressin type 2 receptor; Vasopressin type II receptor; Vasopressin V2 receptor; V2 vasopressin receptor; Vasopressin receptor type 2; DIR; Type 2 vasopressin receptor; Human AXIN1; Axin-1; anti-AXIN1 antibody

• Host: Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Zebrafish
• Clonality: Polyclonal
• Specificity: Human AXIN1. This antibody is expected to recognise both reported isoforms.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5mg/ml (varies by lot)
• Sequence Positions: 850-862

• Applicable Applications for anti-AXIN1 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Target: AXIN / Axin-1
• Immunogen species: AXIN / Axin-1 antibody was raised againist Human
• Immunogen: AXIN / Axin-1 antibody was raised against peptide with sequence C-FEEKIIGKVEKVD, from the C-Terminus of protein sequence accordinf=g to NP_003493, NP_851393.1.
• Usage: Immunohistochemistry: MBS242192 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242192 was determined to be 3.75 ug/ml.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(Anti-AXIN1 / AXIN antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 27501450
• NCBI GeneID: 8312
• NCBI Accession #: NP_003493.1
• NCBI GenBank Nucleotide #: NM_003502.3
• UniProt Accession #: O15169; Q4TT26; Q4TT27; Q86YA7; Q8WVW6; Q96S28
• NCBI Official Full Name: axin-1 isoform a
• NCBI Official Synonym Full Names: axin 1
• NCBI Official Symbol: AXIN1
• NCBI Official Synonym Symbols: AXIN; PPP1R49
• NCBI Protein Information: axin-1; axis inhibitor 1; fused, mouse, homolog of; axis inhibition protein 1; protein phosphatase 1, regulatory subunit 49
• UniProt Protein Name: Axin-1
• UniProt Gene Name: AXIN1
• UniProt Synonym Gene Names: AXIN; hAxin
• UniProt Entry Name: AXIN1_HUMAN