Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate;;Lane 2: Bcl2 knockout Jurkat cell lysate;;Predicted MW: 26kDa ;Observed MW: 28kDa;Primary Ab: 3ug/ml Rabbit Anti-Human Bcl2 Ab;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit B-Cell Leukemia/Lymphoma 2 (Bcl2) Polyclonal Antibody | anti-Bcl2 antibody
Polyclonal Antibody to B-Cell Leukemia/Lymphoma 2 (Bcl2)
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Pig
Applications
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Chromatography
Synonyms
B-Cell Leukemia/Lymphoma 2 (Bcl2), Antibody; Polyclonal Antibody to B-Cell Leukemia/Lymphoma 2 (Bcl2); anti-Bcl2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against Bcl2. It has been selected for its ability to recognize Bcl2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D
Applicable Applications for anti-Bcl2 antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Recombinant Bcl2 (Ala2~Asp211) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate;;Lane 2: Bcl2 knockout Jurkat cell lysate;;Predicted MW: 26kDa ;Observed MW: 28kDa;Primary Ab: 3ug/ml Rabbit Anti-Human Bcl2 Ab;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate;;Lane 2: Bcl2 knockout Jurkat cell lysate;;Predicted MW: 26kDa ;Observed MW: 28kDa;Primary Ab: 3ug/ml Rabbit Anti-Human Bcl2 Ab;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
WB (Western Blot)
(Western Blot: Sample: Recombinant Bcl2, Human.)
WB (Western Blot)
(Western Blot: Sample: Recombinant Bcl2, Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human U937 Cells; Lane3: Mouse Pancreas Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human U937 Cells; Lane3: Mouse Pancreas Tissue.)
Product Categories/Family for anti-Bcl2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,337 Da
NCBI Official Full Name
apoptosis regulator Bcl-2 alpha isoform
NCBI Official Synonym Full Names
BCL2, apoptosis regulator
NCBI Official Symbol
BCL2
NCBI Official Synonym Symbols
Bcl-2; PPP1R50
NCBI Protein Information
apoptosis regulator Bcl-2
UniProt Protein Name
Apoptosis regulator Bcl-2
UniProt Gene Name
BCL2
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Product Notes
The Bcl2 bcl2 (Catalog #AAA131669) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to B-Cell Leukemia/Lymphoma 2 (Bcl2) reacts with Human, Mouse, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's B-Cell Leukemia/Lymphoma 2 (Bcl2) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the Bcl2 bcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D. It is sometimes possible for the material contained within the vial of "B-Cell Leukemia/Lymphoma 2 (Bcl2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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