Rabbit anti-Human, Mouse BAD Polyclonal Antibody | anti-BAD antibody

Rabbit anti-BAD Antibody, Affinity Purified

Cat. Num. AAA211862

• Gene Names: BAD; BBC2; BCL2L8
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: BAD, Antibody; Rabbit anti-BAD Antibody, Affinity Purified; bcl-2-like protein 8; BCL-X/BCL-2 binding protein; BCL2-binding component 6; bcl2-L-8; BCL2L8; BCL2-binding protein; bcl2-associated agonist of cell death; BCL2-antagonist of cell death protein; bcl2 antagonist of cell death; BBC2; BAD; bcl-2-binding component 6; bcl-XL/Bcl-2-associated death promoter; Bcl2-associated agonist of cell death; BCL2 associated agonist of cell death; anti-BAD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-BAD antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation)
• Predicted Reactivity: Rat
• Immunogen: Between 1 and 50
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

IP (Immunoprecipitation)

(Detection of human BAD by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BAD antibody AAA211862 used for IP at 10 ug/mg lysate. BAD was also immunoprecipitated by rabbit anti-BAD antibody BL6706, which recognizes a downstream epitope. For blotting immunoprecipitated BAD, AAA211862 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human and mouse BAD by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-BAD (Cat. No. AAA211862) used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB)

Related Product Information for anti-BAD antibody

BAD (Bcl2 agonist of cell death) is a member of the Bcl-2 family. Bcl-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein [taken from NCBI Entrez Gene (GeneID:572)].

Product Categories/Family for anti-BAD antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 10835069
• NCBI GeneID: 572
• NCBI Accession #: NP_004313.1
• NCBI GenBank Nucleotide #: NM_004322.3
• UniProt Accession #: Q92934; O14803; Q6FH21
• Molecular Weight: 18,392 Da
• NCBI Official Full Name: bcl2 antagonist of cell death
• NCBI Official Synonym Full Names: BCL2-associated agonist of cell death
• NCBI Official Symbol: BAD
• NCBI Official Synonym Symbols: BBC2; BCL2L8
• NCBI Protein Information: bcl2 antagonist of cell death; bcl2-L-8; BCL2-binding protein; bcl-2-like protein 8; BCL2-binding component 6; bcl-2-binding component 6; BCL-X/BCL-2 binding protein; BCL2-antagonist of cell death protein; bcl-XL/Bcl-2-associated death promoter
• UniProt Protein Name: Bcl2 antagonist of cell death
• UniProt Gene Name: BAD
• UniProt Synonym Gene Names: BBC6; BCL2L8; BAD; Bcl2-L-8
• UniProt Entry Name: BAD_HUMAN