Rabbit anti-Human Bad-S118 Polyclonal Antibody | anti-Bad-S118 antibody
Phospho-Bad-S118 Polyclonal Antibody
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
Bad-S118, Antibody; Phospho-Bad-S118 Polyclonal Antibody; BBC2; BCL2L8; anti-Bad-S118 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
168
Applicable Applications for anti-Bad-S118 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A phospho specific peptide corresponding to residues surrounding S118 of human Bad
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20°C or-80°C. Avoid freeze/thaw cycles.
Related Product Information for anti-Bad-S118 antibody
Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1, 2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3, 4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5, 6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).
References
1. Yang, E. et al. (1995) Cell 80, 285-291. 2. Zha, J. et al. (1996) Cell 87, 619-628. 3. Datta, S.R. et al. (1997) Cell 91, 231-241. 4. Peso, L. et al. (1997) Science 278, 687-689. 5. Bonni, A. et al. (1999) Science 286, 1358-1362. 6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867. 7. Harada, H. et al. (1999) Mol. Cell 3, 413-422. 8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869. 9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557. 10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18kDa
NCBI Official Full Name
bcl2-associated agonist of cell death
NCBI Official Synonym Full Names
BCL2 associated agonist of cell death
NCBI Official Symbol
BAD
NCBI Official Synonym Symbols
BBC2; BCL2L8
NCBI Protein Information
bcl2-associated agonist of cell death
UniProt Protein Name
Bcl2-associated agonist of cell death
UniProt Gene Name
BAD
UniProt Synonym Gene Names
BBC6; BCL2L8; BAD; Bcl2-L-8
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Product Notes
The Bad-S118 bad (Catalog #AAA268239) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Bad-S118 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bad-S118 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Bad-S118 bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bad-S118, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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