Rabbit anti-Human, mouse BAI1 Polyclonal Antibody | anti-BAI1 antibody

BAI1 Antibody (C-term)

Cat. Num. AAA289523

• Gene Names: ADGRB1; BAI1; GDAIF
• Reactivity: Human, mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: BAI1, Antibody; BAI1 Antibody (C-term); Brain-specific angiogenesis inhibitor 1; BAI1; anti-BAI1 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This BAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1537-1567 amino acids from the C-terminal region of human BAI1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 1537-1567
• Sequence Length: 1,584

• Applicable Applications for anti-BAI1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Cellular Location: Cell membrane; Multi-pass membrane protein Note=Likely to be concentrated at cell-cell adhesion sites
• Tissue Location: Specifically expressed in brain. Reduced or no expression is observed in some glioblastoma cell lines and cancer tissues. No expression in astrocytes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(BAI1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(BAI1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BAI1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(BAI1 Antibody (Q1552) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the BAI1 antibody detected the BAI1 protein (arrow).)

WB (Western Blot)

(BAI1 Antibody (Q1552) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BAI1 antibody detected the BAI1 protein (arrow).)

Related Product Information for anti-BAI1 antibody

Angiogenesis is controlled by a local balance between stimulators and inhibitors of new vessel growth and is suppressed under normal physiologic conditions. Angiogenesis has been shown to be essential for growth and metastasis of solid tumors. In order to obtain blood supply for their growth, tumor cells are potently angiogenic and attract new vessels as results of increased secretion of inducers and decreased production of endogenous negative regulators. BAI1 contains at least one 'functional' p53-binding site within an intron, and its expression has been shown to be induced by wildtype p53. There are two other brain-specific angiogenesis inhibitor genes, designated BAI2 and BAI3 which along with BAI1 have similar tissue specificities and structures, however only BAI1 is transcriptionally regulated by p53. BAI1 is postulated to be a member of the secretin receptor family, an inhibitor of angiogenesis and a growth suppressor of glioblastomas.

Function: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Likely to be a potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53 signal in suppression of glioblastoma. May function in cell adhesion and signal transduction in the brain.

Product Categories/Family for anti-BAI1 antibody

Cardiovascular; Immunology; GPCR Antibodies

References

Kaur, B., et al., Am. J. Pathol. 162(1):19-27 (2003). Mori, K., et al., Neurosci. Res. 43(1):69-74 (2002). Duda, D.G., et al., Br. J. Cancer 86(3):490-496 (2002). Shiratsuchi, T., et al., Biochem. Biophys. Res. Commun. 247(3):597-604 (1998). Fukushima, Y., et al., Int. J. Oncol. 13(5):967-970 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 111118994
• NCBI GeneID: 575
• NCBI Accession #: NP_001693.2
• NCBI GenBank Nucleotide #: NM_001702.2
• UniProt Accession #: O14514
• Molecular Weight: Calculated MW: 173501
• NCBI Official Full Name: brain-specific angiogenesis inhibitor 1
• NCBI Official Synonym Full Names: adhesion G protein-coupled receptor B1
• NCBI Official Symbol: ADGRB1
• NCBI Official Synonym Symbols: BAI1; GDAIF
• NCBI Protein Information: brain-specific angiogenesis inhibitor 1
• UniProt Protein Name: Brain-specific angiogenesis inhibitor 1
• UniProt Gene Name: BAI1
• UniProt Entry Name: BAI1_HUMAN