WB (Western Blot)
(Detection of human BAP1 by western blot. Samples: Whole cell lysate (50 ug) from RKO, HeLa, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAP1 antibody (AAA211816 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human BAP1 Polyclonal Antibody | anti-BAP1 antibody
Rabbit anti-BAP1 Antibody, Affinity Purified
Gene Names
BAP1; UCHL2; hucep-6; HUCEP-13
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BAP1, Antibody; Rabbit anti-BAP1 Antibody, Affinity Purified; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); UCHL2; ubiquitin carboxyl-terminal hydrolase BAP1; hucep-6; HUCEP-13; cerebral protein-13; BRCA1-associated protein 1; cerebral protein 6; Ubiquitin carboxyl-terminal hydrolase BAP1; BRCA1 associated protein 1; anti-BAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BAP1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Zebrafish, X. tropicalis, Chicken, Bovine
Immunogen
Between 679 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human BAP1 by western blot. Samples: Whole cell lysate (50 ug) from RKO, HeLa, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAP1 antibody (AAA211816 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human BAP1 by western blot. Samples: Whole cell lysate (50 ug) from RKO, HeLa, Hep-G2, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAP1 antibody (AAA211816 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human BAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAP1 antibody (AAA211816 lot 2) used for IP at 6 ug per reaction. BAP1 was also immunoprecipitated by a previous lot of this antibody (AAA211816 lot 1) and a second antibody against a different epitope of BAP1 . For blotting immunoprecipitated BAP1, AAA211816 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human BAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BAP1 antibody (AAA211816 lot 2) used for IP at 6 ug per reaction. BAP1 was also immunoprecipitated by a previous lot of this antibody (AAA211816 lot 1) and a second antibody against a different epitope of BAP1 . For blotting immunoprecipitated BAP1, AAA211816 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human BAP1 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-BAP1 antibody (AAA211816). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human BAP1 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-BAP1 antibody (AAA211816). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for anti-BAP1 antibody
BRCA1-associated protein 1 (BAP1) functions as a C-terminal ubiquitin hydrolase and may be involved in the transduction of signals in the BRCA1 pathway. BAP1 is also implicated to be a tumor suppressor as defects in BAP1 are found in non-small cell lung cancer cell lines.
Product Categories/Family for anti-BAP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,362 Da
NCBI Official Full Name
Homo sapiens BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (BAP1), mRNA
NCBI Official Synonym Full Names
BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
NCBI Official Symbol
BAP1
NCBI Official Synonym Symbols
UCHL2; hucep-6; HUCEP-13
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase BAP1; cerebral protein 6; cerebral protein-13
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase BAP1
UniProt Gene Name
BAP1
UniProt Synonym Gene Names
KIAA0272
UniProt Entry Name
BAP1_HUMAN
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Product Notes
The BAP1 bap1 (Catalog #AAA211816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BAP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BAP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BAP1 bap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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