Rabbit BARON Polyclonal Antibody | anti-KIAA0226 antibody

BARON Antibody (Center)

Cat. Num. AAA290675

• Gene Names: RUBCN; SCAR15; RUBICON; KIAA0226
• Reactivity: Human; Predicted Reactivity: Mouse
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: BARON, Antibody; BARON Antibody (Center); Run domain Beclin-1 interacting and cysteine-rich containing protein; Rubicon; Beclin-1 associated RUN domain containing protein; Baron; KIAA0226; anti-KIAA0226 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the Central region of human BARON.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 927

• Applicable Applications for anti-KIAA0226 antibody: ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Late endosome. Lysosome. Early endosome. Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus.
• Antigen Region: 656-684 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(BARON Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human kidney reacted with BARON Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(All lanes : Anti-BARON Antibody (Center) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: Raji whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-KIAA0226 antibody

Primary Antibodies; Cancer; Cardiovascular; Cell Biology; Metabolism

References

Matsunaga, K., et al. Nat. Cell Biol. 11 (4):385-396 (2009). Muzny, D.M., et al. Nature 440 (7088):1194-1198 (2006). Venter, J.C., et al. Science 291 (5507):1304-1351 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 224548938
• NCBI GeneID: 9711
• NCBI Accession #: NP_001139114.1
• NCBI GenBank Nucleotide #: NM_001145642.3
• UniProt Accession #: Q92622; Q96CK5
• Molecular Weight: 108622 Da
• NCBI Official Full Name: run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 1
• NCBI Official Synonym Full Names: RUN and cysteine rich domain containing beclin 1 interacting protein
• NCBI Official Symbol: RUBCN
• NCBI Official Synonym Symbols: SCAR15; RUBICON; KIAA0226
• NCBI Protein Information: run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• UniProt Protein Name: Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
• UniProt Gene Name: RUBCN
• UniProt Synonym Gene Names: Rubicon; Baron
• UniProt Entry Name: RUBIC_HUMAN