Rabbit Rubicon Polyclonal Antibody | anti-KIAA0226 antibody

Rubicon Antibody

Cat. Num. AAA40538

• Gene Names: KIAA0226; SCAR15; RUBICON
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Rubicon antibody is affinity chromatography purified via peptide column.
• Synonyms: Rubicon, Antibody; Rubicon Antibody; Run domain Beclin-1 interacting and cysteine-rich containing protein; KIAA0226; anti-KIAA0226 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
• Purity/Purification: Rubicon antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 987

• Applicable Applications for anti-KIAA0226 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.; The immunogen is located within amino acids 240-290 of Rubicon.
• Buffer: Rubicon antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Rubicon antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 μg/ml.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 μg/ml.)

WB (Western Blot)

(Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 μg/ml.)

Related Product Information for anti-KIAA0226 antibody

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

NCBI and Uniprot Product Information

• NCBI GI #: 530375960
• NCBI GeneID: 9711
• NCBI Accession #: XP_005269431
• NCBI GenBank Nucleotide #: XM_005269374.1
• Molecular Weight: Predicted: 90; 101; 109 kDa; ; Observed: 91; 99 kDa
• NCBI Official Full Name: run domain Beclin-1 interacting and cysteine-rich containing protein isoform X1
• NCBI Official Synonym Full Names: KIAA0226
• NCBI Official Symbol: KIAA0226
• NCBI Official Synonym Symbols: SCAR15; RUBICON
• NCBI Protein Information: run domain Beclin-1 interacting and cysteine-rich containing protein; baron; rundataxin; beclin-1 associated RUN domain containing protein; run domain Beclin-1 interacting and cystein-rich containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
• UniProt Protein Name: Run domain Beclin-1 interacting and cysteine-rich containing protein
• UniProt Gene Name: KIAA0226
• UniProt Synonym Gene Names: Rubicon; Baron
• UniProt Entry Name: RUBIC_HUMAN