FCM/FACS (Flow Cytometry)
(BAT1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit BAT1 Polyclonal Antibody | anti-DDX39B antibody
BAT1 Antibody (C-term)
Gene Names
DDX39B; BAT1; UAP56; D6S81E
Reactivity
Human (Predicted Reactivity: Mouse, Rat, Bovine, Chicken, Drosophila, Pig)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BAT1, Antibody; BAT1 Antibody (C-term); Spliceosome RNA helicase DDX39B; 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD box protein UAP56; HLA-B-associated transcript 1 protein; DDX39B; BAT1; UAP56; anti-DDX39B antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat, Bovine, Chicken, Drosophila, Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from the C-terminal region of human BAT1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
351-380
Sequence Length
428
Applicable Applications for anti-DDX39B antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(BAT1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(BAT1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human kidney reacted with BAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human kidney reacted with BAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(BAT1 Antibody (C-term) western blot analysis in A431,Hela,Jurkat cell line lysates (35ug/lane).This demonstrates the BAT1 antibody detected the BAT1 protein (arrow).)
WB (Western Blot)
(BAT1 Antibody (C-term) western blot analysis in A431,Hela,Jurkat cell line lysates (35ug/lane).This demonstrates the BAT1 antibody detected the BAT1 protein (arrow).)
Related Product Information for anti-DDX39B antibody
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. It has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. It can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. It can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.
Product Categories/Family for anti-DDX39B antibody
References
Choudhary C., et.al., Science 325:834-840(2009).
Boyne J.R., et.al., PLoS Pathog. 4:E1000194-E1000194(2008).
Boyne J.R., et.al., PLoS Pathog. 4:E1000194-E1000194(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48991
NCBI Official Full Name
spliceosome RNA helicase DDX39B
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
NCBI Official Symbol
DDX39B
NCBI Official Synonym Symbols
BAT1; UAP56; D6S81E
NCBI Protein Information
spliceosome RNA helicase DDX39B
UniProt Protein Name
Spliceosome RNA helicase DDX39B
UniProt Gene Name
DDX39B
UniProt Synonym Gene Names
BAT1; UAP56
UniProt Entry Name
DX39B_HUMAN
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Product Notes
The DDX39B ddx39b (Catalog #AAA288749) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 351-380. The BAT1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Rat, Bovine, Chicken, Drosophila, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's BAT1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the DDX39B ddx39b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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